ssr_primer
Primer Details for TRINITY_DN13530_c3_g11_i1 |
|---|
| (A)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGCCAAAAGAAAGCTGGTC | ATCGTCGAAATCGGAGTCGG | Start: 92 |
| Tm | 60.039 | 59.971 | End: 213 |
| Size | 20 | 20 | 122 |
| Primer 2 details |
|---|
| Sequence | CCGACTCCGATTTCGACGAT | AACCGAGGCCATGGACAAAT | Start: 194 |
| Tm | 59.971 | 59.96 | End: 364 |
| Size | 20 | 20 | 171 |
| Primer 3 details |
|---|
| Sequence | GTCCGCCGAAACCTTGTTTC | ATCGTCGAAATCGGAGTCGG | Start: 2 |
| Tm | 60.041 | 59.971 | End: 213 |
| Size | 20 | 20 | 212 |
| Primer 4 details |
|---|
| Sequence | CGAACCCTTCTCCAAGACCC | TAATTGCACCTTGCCGGGAT | Start: 228 |
| Tm | 60.036 | 60.034 | End: 505 |
| Size | 20 | 20 | 278 |
| Primer 5 details |
|---|
| Sequence | ATTTGTCCATGGCCTCGGTT | TAATTGCACCTTGCCGGGAT | Start: 345 |
| Tm | 59.96 | 60.034 | End: 505 |
| Size | 20 | 20 | 161 |
(TA)15tttatttg(TA)9: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGCCAAAAGAAAGCTGGTC | ATCGTCGAAATCGGAGTCGG | Start: 92 |
| Tm | 60.039 | 59.971 | End: 213 |
| Size | 20 | 20 | 122 |
| Primer 2 details |
|---|
| Sequence | CCGACTCCGATTTCGACGAT | AACCGAGGCCATGGACAAAT | Start: 194 |
| Tm | 59.971 | 59.96 | End: 364 |
| Size | 20 | 20 | 171 |
| Primer 3 details |
|---|
| Sequence | GTCCGCCGAAACCTTGTTTC | ATCGTCGAAATCGGAGTCGG | Start: 2 |
| Tm | 60.041 | 59.971 | End: 213 |
| Size | 20 | 20 | 212 |
| Primer 4 details |
|---|
| Sequence | CGAACCCTTCTCCAAGACCC | TAATTGCACCTTGCCGGGAT | Start: 228 |
| Tm | 60.036 | 60.034 | End: 505 |
| Size | 20 | 20 | 278 |
| Primer 5 details |
|---|
| Sequence | ATTTGTCCATGGCCTCGGTT | TAATTGCACCTTGCCGGGAT | Start: 345 |
| Tm | 59.96 | 60.034 | End: 505 |
| Size | 20 | 20 | 161 |
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