ssr_primer
Primer Details for TRINITY_DN13527_c2_g3_i4 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGCTGACTTGGGGCTTTTT | ACACACAGCTGGGACCAAAA | Start: 3136 |
| Tm | 60.106 | 60.034 | End: 3359 |
| Size | 20 | 20 | 224 |
| Primer 2 details |
|---|
| Sequence | AGGCTGACTTGGGGCTTTTT | AACACACAGCTGGGACCAAA | Start: 3136 |
| Tm | 60.106 | 60.034 | End: 3360 |
| Size | 20 | 20 | 225 |
| Primer 3 details |
|---|
| Sequence | AGGCTGACTTGGGGCTTTTT | AAACACACAGCTGGGACCAA | Start: 3136 |
| Tm | 60.106 | 60.034 | End: 3361 |
| Size | 20 | 20 | 226 |
| Primer 4 details |
|---|
| Sequence | AGGCTGACTTGGGGCTTTTT | AAAACACACAGCTGGGACCA | Start: 3136 |
| Tm | 60.106 | 60.034 | End: 3362 |
| Size | 20 | 20 | 227 |
| Primer 5 details |
|---|
| Sequence | GATCCTCCTTTTGGCAGGCT | TGCCGAGTTGAACTGCTCTT | Start: 2433 |
| Tm | 60.034 | 59.893 | End: 2590 |
| Size | 20 | 20 | 158 |
(A)11caaaaacaagaagaagat(GAA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGCTGACTTGGGGCTTTTT | ACACACAGCTGGGACCAAAA | Start: 3136 |
| Tm | 60.106 | 60.034 | End: 3359 |
| Size | 20 | 20 | 224 |
| Primer 2 details |
|---|
| Sequence | AGGCTGACTTGGGGCTTTTT | AACACACAGCTGGGACCAAA | Start: 3136 |
| Tm | 60.106 | 60.034 | End: 3360 |
| Size | 20 | 20 | 225 |
| Primer 3 details |
|---|
| Sequence | AGGCTGACTTGGGGCTTTTT | AAACACACAGCTGGGACCAA | Start: 3136 |
| Tm | 60.106 | 60.034 | End: 3361 |
| Size | 20 | 20 | 226 |
| Primer 4 details |
|---|
| Sequence | AGGCTGACTTGGGGCTTTTT | AAAACACACAGCTGGGACCA | Start: 3136 |
| Tm | 60.106 | 60.034 | End: 3362 |
| Size | 20 | 20 | 227 |
| Primer 5 details |
|---|
| Sequence | GATCCTCCTTTTGGCAGGCT | TGCCGAGTTGAACTGCTCTT | Start: 2433 |
| Tm | 60.034 | 59.893 | End: 2590 |
| Size | 20 | 20 | 158 |
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