Primer Details for TRINITY_DN13514_c5_g1_i1 |
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| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTTTTGTAATTGTAGCCTTTCTATGA | CAACCTAAGGGCAAGGGACA | Start: 3 |
| Tm | 57.485 | 59.596 | End: 103 |
| Size | 27 | 20 | 101 |
| Primer 2 details |
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| Sequence | ACTTTTGTAATTGTAGCCTTTCTATGA | ACAACCTAAGGGCAAGGGAC | Start: 3 |
| Tm | 57.485 | 59.596 | End: 104 |
| Size | 27 | 20 | 102 |
| Primer 3 details |
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| Sequence | TGTCCCTTGCCCTTAGGTTG | ACAAGATAGTTATACAAAGAGAGCAAA | Start: 84 |
| Tm | 59.596 | 57.056 | End: 186 |
| Size | 20 | 27 | 103 |
| Primer 4 details |
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| Sequence | GTCCCTTGCCCTTAGGTTGT | ACAAGATAGTTATACAAAGAGAGCAAA | Start: 85 |
| Tm | 59.596 | 57.056 | End: 186 |
| Size | 20 | 27 | 102 |
| Primer 5 details |
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| Sequence | ACTTTTGTAATTGTAGCCTTTCTATGA | GAACAACCTAAGGGCAAGGGA | Start: 3 |
| Tm | 57.485 | 59.925 | End: 106 |
| Size | 27 | 21 | 104 |
