ssr_primer
Primer Details for TRINITY_DN13501_c1_g4_i9 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCAGTTGTTTGGCTTCAGC | AGGAAGATTTGGGCGTGAGG | Start: 2541 |
| Tm | 59.967 | 60.035 | End: 2742 |
| Size | 20 | 20 | 202 |
| Primer 2 details |
|---|
| Sequence | CCCAGTTGTTTGGCTTCAGC | GAAGATTTGGGCGTGAGGGA | Start: 2541 |
| Tm | 59.967 | 60.035 | End: 2740 |
| Size | 20 | 20 | 200 |
| Primer 3 details |
|---|
| Sequence | CCGGTGCTTTGTCATCTCCT | TGCTGTGAAGGTTCCGGATC | Start: 5206 |
| Tm | 60.036 | 60.036 | End: 5374 |
| Size | 20 | 20 | 169 |
| Primer 4 details |
|---|
| Sequence | CCGGTGCTTTGTCATCTCCT | ACGAGGAAGACGGAGGAAGA | Start: 5206 |
| Tm | 60.036 | 59.962 | End: 5326 |
| Size | 20 | 20 | 121 |
| Primer 5 details |
|---|
| Sequence | TCTCAACTCTCCGGCCAAAC | AAAATCGCGGCAAACAGTCC | Start: 4844 |
| Tm | 59.965 | 60.04 | End: 4944 |
| Size | 20 | 20 | 101 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCAGTTGTTTGGCTTCAGC | AGGAAGATTTGGGCGTGAGG | Start: 2541 |
| Tm | 59.967 | 60.035 | End: 2742 |
| Size | 20 | 20 | 202 |
| Primer 2 details |
|---|
| Sequence | CCCAGTTGTTTGGCTTCAGC | GAAGATTTGGGCGTGAGGGA | Start: 2541 |
| Tm | 59.967 | 60.035 | End: 2740 |
| Size | 20 | 20 | 200 |
| Primer 3 details |
|---|
| Sequence | CCGGTGCTTTGTCATCTCCT | TGCTGTGAAGGTTCCGGATC | Start: 5206 |
| Tm | 60.036 | 60.036 | End: 5374 |
| Size | 20 | 20 | 169 |
| Primer 4 details |
|---|
| Sequence | CCGGTGCTTTGTCATCTCCT | ACGAGGAAGACGGAGGAAGA | Start: 5206 |
| Tm | 60.036 | 59.962 | End: 5326 |
| Size | 20 | 20 | 121 |
| Primer 5 details |
|---|
| Sequence | TCTCAACTCTCCGGCCAAAC | AAAATCGCGGCAAACAGTCC | Start: 4844 |
| Tm | 59.965 | 60.04 | End: 4944 |
| Size | 20 | 20 | 101 |
(A)10gaaaaaaccatcgaaaaatcgatatatcatgaacctcgatccaaacacaacgatccgatcaaccagaaaattccagggataaaacg(A)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCAGTTGTTTGGCTTCAGC | AGGAAGATTTGGGCGTGAGG | Start: 2541 |
| Tm | 59.967 | 60.035 | End: 2742 |
| Size | 20 | 20 | 202 |
| Primer 2 details |
|---|
| Sequence | CCCAGTTGTTTGGCTTCAGC | GAAGATTTGGGCGTGAGGGA | Start: 2541 |
| Tm | 59.967 | 60.035 | End: 2740 |
| Size | 20 | 20 | 200 |
| Primer 3 details |
|---|
| Sequence | CCGGTGCTTTGTCATCTCCT | TGCTGTGAAGGTTCCGGATC | Start: 5206 |
| Tm | 60.036 | 60.036 | End: 5374 |
| Size | 20 | 20 | 169 |
| Primer 4 details |
|---|
| Sequence | CCGGTGCTTTGTCATCTCCT | ACGAGGAAGACGGAGGAAGA | Start: 5206 |
| Tm | 60.036 | 59.962 | End: 5326 |
| Size | 20 | 20 | 121 |
| Primer 5 details |
|---|
| Sequence | TCTCAACTCTCCGGCCAAAC | AAAATCGCGGCAAACAGTCC | Start: 4844 |
| Tm | 59.965 | 60.04 | End: 4944 |
| Size | 20 | 20 | 101 |
(GA)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCAGTTGTTTGGCTTCAGC | AGGAAGATTTGGGCGTGAGG | Start: 2541 |
| Tm | 59.967 | 60.035 | End: 2742 |
| Size | 20 | 20 | 202 |
| Primer 2 details |
|---|
| Sequence | CCCAGTTGTTTGGCTTCAGC | GAAGATTTGGGCGTGAGGGA | Start: 2541 |
| Tm | 59.967 | 60.035 | End: 2740 |
| Size | 20 | 20 | 200 |
| Primer 3 details |
|---|
| Sequence | CCGGTGCTTTGTCATCTCCT | TGCTGTGAAGGTTCCGGATC | Start: 5206 |
| Tm | 60.036 | 60.036 | End: 5374 |
| Size | 20 | 20 | 169 |
| Primer 4 details |
|---|
| Sequence | CCGGTGCTTTGTCATCTCCT | ACGAGGAAGACGGAGGAAGA | Start: 5206 |
| Tm | 60.036 | 59.962 | End: 5326 |
| Size | 20 | 20 | 121 |
| Primer 5 details |
|---|
| Sequence | TCTCAACTCTCCGGCCAAAC | AAAATCGCGGCAAACAGTCC | Start: 4844 |
| Tm | 59.965 | 60.04 | End: 4944 |
| Size | 20 | 20 | 101 |
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