ssr_primer
Primer Details for TRINITY_DN13493_c2_g4_i3 |
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| (A)12ggttaactaatgaagagattccttgtacaagtactacacgctgttttccatttctctcttgtggccc(AAAG)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAAACAGTGTACGCACCAG | GGTGTCGGTTGAAGTGAGCT | Start: 842 |
| Tm | 60.041 | 60.25 | End: 1099 |
| Size | 20 | 20 | 258 |
| Primer 2 details |
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| Sequence | GCAAACAGTGTACGCACCAG | AGGTGTCGGTTGAAGTGAGC | Start: 842 |
| Tm | 60.041 | 60.25 | End: 1100 |
| Size | 20 | 20 | 259 |
| Primer 3 details |
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| Sequence | GCTCACTTCAACCGACACCT | CCCTCAACCTCGATCACCTG | Start: 1081 |
| Tm | 60.25 | 59.823 | End: 1201 |
| Size | 20 | 20 | 121 |
| Primer 4 details |
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| Sequence | AGCTCACTTCAACCGACACC | CCCTCAACCTCGATCACCTG | Start: 1080 |
| Tm | 60.25 | 59.823 | End: 1201 |
| Size | 20 | 20 | 122 |
| Primer 5 details |
|---|
| Sequence | AACAGTGTACGCACCAGGTT | GGTGTCGGTTGAAGTGAGCT | Start: 845 |
| Tm | 59.821 | 60.25 | End: 1099 |
| Size | 20 | 20 | 255 |
(TC)8: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAAACAGTGTACGCACCAG | GGTGTCGGTTGAAGTGAGCT | Start: 842 |
| Tm | 60.041 | 60.25 | End: 1099 |
| Size | 20 | 20 | 258 |
| Primer 2 details |
|---|
| Sequence | GCAAACAGTGTACGCACCAG | AGGTGTCGGTTGAAGTGAGC | Start: 842 |
| Tm | 60.041 | 60.25 | End: 1100 |
| Size | 20 | 20 | 259 |
| Primer 3 details |
|---|
| Sequence | GCTCACTTCAACCGACACCT | CCCTCAACCTCGATCACCTG | Start: 1081 |
| Tm | 60.25 | 59.823 | End: 1201 |
| Size | 20 | 20 | 121 |
| Primer 4 details |
|---|
| Sequence | AGCTCACTTCAACCGACACC | CCCTCAACCTCGATCACCTG | Start: 1080 |
| Tm | 60.25 | 59.823 | End: 1201 |
| Size | 20 | 20 | 122 |
| Primer 5 details |
|---|
| Sequence | AACAGTGTACGCACCAGGTT | GGTGTCGGTTGAAGTGAGCT | Start: 845 |
| Tm | 59.821 | 60.25 | End: 1099 |
| Size | 20 | 20 | 255 |
(ATT)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAAACAGTGTACGCACCAG | GGTGTCGGTTGAAGTGAGCT | Start: 842 |
| Tm | 60.041 | 60.25 | End: 1099 |
| Size | 20 | 20 | 258 |
| Primer 2 details |
|---|
| Sequence | GCAAACAGTGTACGCACCAG | AGGTGTCGGTTGAAGTGAGC | Start: 842 |
| Tm | 60.041 | 60.25 | End: 1100 |
| Size | 20 | 20 | 259 |
| Primer 3 details |
|---|
| Sequence | GCTCACTTCAACCGACACCT | CCCTCAACCTCGATCACCTG | Start: 1081 |
| Tm | 60.25 | 59.823 | End: 1201 |
| Size | 20 | 20 | 121 |
| Primer 4 details |
|---|
| Sequence | AGCTCACTTCAACCGACACC | CCCTCAACCTCGATCACCTG | Start: 1080 |
| Tm | 60.25 | 59.823 | End: 1201 |
| Size | 20 | 20 | 122 |
| Primer 5 details |
|---|
| Sequence | AACAGTGTACGCACCAGGTT | GGTGTCGGTTGAAGTGAGCT | Start: 845 |
| Tm | 59.821 | 60.25 | End: 1099 |
| Size | 20 | 20 | 255 |
(TA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAAACAGTGTACGCACCAG | GGTGTCGGTTGAAGTGAGCT | Start: 842 |
| Tm | 60.041 | 60.25 | End: 1099 |
| Size | 20 | 20 | 258 |
| Primer 2 details |
|---|
| Sequence | GCAAACAGTGTACGCACCAG | AGGTGTCGGTTGAAGTGAGC | Start: 842 |
| Tm | 60.041 | 60.25 | End: 1100 |
| Size | 20 | 20 | 259 |
| Primer 3 details |
|---|
| Sequence | GCTCACTTCAACCGACACCT | CCCTCAACCTCGATCACCTG | Start: 1081 |
| Tm | 60.25 | 59.823 | End: 1201 |
| Size | 20 | 20 | 121 |
| Primer 4 details |
|---|
| Sequence | AGCTCACTTCAACCGACACC | CCCTCAACCTCGATCACCTG | Start: 1080 |
| Tm | 60.25 | 59.823 | End: 1201 |
| Size | 20 | 20 | 122 |
| Primer 5 details |
|---|
| Sequence | AACAGTGTACGCACCAGGTT | GGTGTCGGTTGAAGTGAGCT | Start: 845 |
| Tm | 59.821 | 60.25 | End: 1099 |
| Size | 20 | 20 | 255 |
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