ssr_primer
Primer Details for TRINITY_DN13491_c1_g3_i8 |
|---|
| (GA)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTACATGACGCCTCTCCCT | CCAAAGACAAGCCAAAGCCC | Start: 4640 |
| Tm | 60.033 | 59.966 | End: 4835 |
| Size | 20 | 20 | 196 |
| Primer 2 details |
|---|
| Sequence | GACCAGTTGCTTTCTCCCGA | AGGGAGAGGCGTCATGTACT | Start: 4446 |
| Tm | 59.965 | 60.033 | End: 4659 |
| Size | 20 | 20 | 214 |
| Primer 3 details |
|---|
| Sequence | GTTCAACCTGGCCTCTTCGA | AGGGAGAGGCGTCATGTACT | Start: 4386 |
| Tm | 59.965 | 60.033 | End: 4659 |
| Size | 20 | 20 | 274 |
| Primer 4 details |
|---|
| Sequence | GACCTCAGCATCAGCCAACT | CCAAAGACAAGCCAAAGCCC | Start: 4612 |
| Tm | 60.036 | 59.966 | End: 4835 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
|---|
| Sequence | AGACCTCAGCATCAGCCAAC | CCAAAGACAAGCCAAAGCCC | Start: 4611 |
| Tm | 60.036 | 59.966 | End: 4835 |
| Size | 20 | 20 | 225 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTACATGACGCCTCTCCCT | CCAAAGACAAGCCAAAGCCC | Start: 4640 |
| Tm | 60.033 | 59.966 | End: 4835 |
| Size | 20 | 20 | 196 |
| Primer 2 details |
|---|
| Sequence | GACCAGTTGCTTTCTCCCGA | AGGGAGAGGCGTCATGTACT | Start: 4446 |
| Tm | 59.965 | 60.033 | End: 4659 |
| Size | 20 | 20 | 214 |
| Primer 3 details |
|---|
| Sequence | GTTCAACCTGGCCTCTTCGA | AGGGAGAGGCGTCATGTACT | Start: 4386 |
| Tm | 59.965 | 60.033 | End: 4659 |
| Size | 20 | 20 | 274 |
| Primer 4 details |
|---|
| Sequence | GACCTCAGCATCAGCCAACT | CCAAAGACAAGCCAAAGCCC | Start: 4612 |
| Tm | 60.036 | 59.966 | End: 4835 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
|---|
| Sequence | AGACCTCAGCATCAGCCAAC | CCAAAGACAAGCCAAAGCCC | Start: 4611 |
| Tm | 60.036 | 59.966 | End: 4835 |
| Size | 20 | 20 | 225 |
(A)11ccatcgtgtaataaagcatttcaatccacattctccacaaaatttgacaaaaataaattt(A)12ccaaaccagatcaatcagctcaaattaaaag(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTACATGACGCCTCTCCCT | CCAAAGACAAGCCAAAGCCC | Start: 4640 |
| Tm | 60.033 | 59.966 | End: 4835 |
| Size | 20 | 20 | 196 |
| Primer 2 details |
|---|
| Sequence | GACCAGTTGCTTTCTCCCGA | AGGGAGAGGCGTCATGTACT | Start: 4446 |
| Tm | 59.965 | 60.033 | End: 4659 |
| Size | 20 | 20 | 214 |
| Primer 3 details |
|---|
| Sequence | GTTCAACCTGGCCTCTTCGA | AGGGAGAGGCGTCATGTACT | Start: 4386 |
| Tm | 59.965 | 60.033 | End: 4659 |
| Size | 20 | 20 | 274 |
| Primer 4 details |
|---|
| Sequence | GACCTCAGCATCAGCCAACT | CCAAAGACAAGCCAAAGCCC | Start: 4612 |
| Tm | 60.036 | 59.966 | End: 4835 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
|---|
| Sequence | AGACCTCAGCATCAGCCAAC | CCAAAGACAAGCCAAAGCCC | Start: 4611 |
| Tm | 60.036 | 59.966 | End: 4835 |
| Size | 20 | 20 | 225 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTACATGACGCCTCTCCCT | CCAAAGACAAGCCAAAGCCC | Start: 4640 |
| Tm | 60.033 | 59.966 | End: 4835 |
| Size | 20 | 20 | 196 |
| Primer 2 details |
|---|
| Sequence | GACCAGTTGCTTTCTCCCGA | AGGGAGAGGCGTCATGTACT | Start: 4446 |
| Tm | 59.965 | 60.033 | End: 4659 |
| Size | 20 | 20 | 214 |
| Primer 3 details |
|---|
| Sequence | GTTCAACCTGGCCTCTTCGA | AGGGAGAGGCGTCATGTACT | Start: 4386 |
| Tm | 59.965 | 60.033 | End: 4659 |
| Size | 20 | 20 | 274 |
| Primer 4 details |
|---|
| Sequence | GACCTCAGCATCAGCCAACT | CCAAAGACAAGCCAAAGCCC | Start: 4612 |
| Tm | 60.036 | 59.966 | End: 4835 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
|---|
| Sequence | AGACCTCAGCATCAGCCAAC | CCAAAGACAAGCCAAAGCCC | Start: 4611 |
| Tm | 60.036 | 59.966 | End: 4835 |
| Size | 20 | 20 | 225 |
(GAA)5gcagtgaagaagaggaacactctgtcttcatgattcggatgccccattttttttcttctccttttctttctttcttcttttagtttag(T)29: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTACATGACGCCTCTCCCT | CCAAAGACAAGCCAAAGCCC | Start: 4640 |
| Tm | 60.033 | 59.966 | End: 4835 |
| Size | 20 | 20 | 196 |
| Primer 2 details |
|---|
| Sequence | GACCAGTTGCTTTCTCCCGA | AGGGAGAGGCGTCATGTACT | Start: 4446 |
| Tm | 59.965 | 60.033 | End: 4659 |
| Size | 20 | 20 | 214 |
| Primer 3 details |
|---|
| Sequence | GTTCAACCTGGCCTCTTCGA | AGGGAGAGGCGTCATGTACT | Start: 4386 |
| Tm | 59.965 | 60.033 | End: 4659 |
| Size | 20 | 20 | 274 |
| Primer 4 details |
|---|
| Sequence | GACCTCAGCATCAGCCAACT | CCAAAGACAAGCCAAAGCCC | Start: 4612 |
| Tm | 60.036 | 59.966 | End: 4835 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
|---|
| Sequence | AGACCTCAGCATCAGCCAAC | CCAAAGACAAGCCAAAGCCC | Start: 4611 |
| Tm | 60.036 | 59.966 | End: 4835 |
| Size | 20 | 20 | 225 |
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