Primer Details for TRINITY_DN13490_c3_g1_i1 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGAAGCAAGAAAGCGCTCC | AGGATGGAGATAGTAAGGCTAACT | Start: 14 |
| Tm | 59.832 | 58.029 | End: 292 |
| Size | 20 | 24 | 279 |
| Primer 2 details | |||
| Sequence | TGAAGCAAGAAAGCGCTCCT | AGGATGGAGATAGTAAGGCTAACT | Start: 15 |
| Tm | 60.25 | 58.029 | End: 292 |
| Size | 20 | 24 | 278 |
| Primer 3 details | |||
| Sequence | GCTGAAGCAAGAAAGCGCTC | AGGATGGAGATAGTAAGGCTAACT | Start: 13 |
| Tm | 60.454 | 58.029 | End: 292 |
| Size | 20 | 24 | 280 |
| Primer 4 details | |||
| Sequence | CTGAAGCAAGAAAGCGCTCC | AGGATGGAGATAGTAAGGCTAACTT | Start: 14 |
| Tm | 59.832 | 58.614 | End: 292 |
| Size | 20 | 25 | 279 |
| Primer 5 details | |||
| Sequence | TGAAGCAAGAAAGCGCTCCT | AGGATGGAGATAGTAAGGCTAACTT | Start: 15 |
| Tm | 60.25 | 58.614 | End: 292 |
| Size | 20 | 25 | 278 |
