Primer Details for TRINITY_DN13482_c0_g2_i1 |
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| (T)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACAAACCAATTTCAACAAACCCT | ACCATGGAAAGTAAGTTGAACAGT | Start: 62 |
| Tm | 57.827 | 58.554 | End: 165 |
| Size | 23 | 24 | 104 |
| Primer 2 details |
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| Sequence | ACAACAAACCAATTTCAACAAACCC | ACCATGGAAAGTAAGTTGAACAGT | Start: 59 |
| Tm | 59.818 | 58.554 | End: 165 |
| Size | 25 | 24 | 107 |
| Primer 3 details |
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| Sequence | CAACAAACCAATTTCAACAAACCCT | ACCATGGAAAGTAAGTTGAACAGT | Start: 60 |
| Tm | 59.586 | 58.554 | End: 165 |
| Size | 25 | 24 | 106 |
| Primer 4 details |
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| Sequence | AACAAACCAATTTCAACAAACCCT | ACCATGGAAAGTAAGTTGAACAGT | Start: 61 |
| Tm | 58.434 | 58.554 | End: 165 |
| Size | 24 | 24 | 105 |
| Primer 5 details |
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| Sequence | ACAAACCAATTTCAACAAACCCT | AACCATGGAAAGTAAGTTGAACAGT | Start: 62 |
| Tm | 57.827 | 59.108 | End: 166 |
| Size | 23 | 25 | 105 |
