ssr_primer
Primer Details for TRINITY_DN13480_c0_g1_i3 |
|---|
| (A)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCAGCATTATGCCCGACAA | CAAGACCACGAAAGCGCAAA | Start: 5289 |
| Tm | 60.035 | 59.971 | End: 5416 |
| Size | 20 | 20 | 128 |
| Primer 2 details |
|---|
| Sequence | ATGGGTGCCGCTATCGTTAG | CCGTCGGCTGCAAAAAGAAA | Start: 3661 |
| Tm | 59.966 | 59.97 | End: 3832 |
| Size | 20 | 20 | 172 |
| Primer 3 details |
|---|
| Sequence | ACGTTGACCAAGATGACCCC | GGTGATCTTGGAGTTGGGCA | Start: 7058 |
| Tm | 59.964 | 59.962 | End: 7297 |
| Size | 20 | 20 | 240 |
| Primer 4 details |
|---|
| Sequence | ACGTTGACCAAGATGACCCC | TGGTGATCTTGGAGTTGGGC | Start: 7058 |
| Tm | 59.964 | 59.962 | End: 7298 |
| Size | 20 | 20 | 241 |
| Primer 5 details |
|---|
| Sequence | ACCAGCATTATGCCCGACAA | CTGCACAAGACCACGAAAGC | Start: 5289 |
| Tm | 60.035 | 60.041 | End: 5421 |
| Size | 20 | 20 | 133 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCAGCATTATGCCCGACAA | CAAGACCACGAAAGCGCAAA | Start: 5289 |
| Tm | 60.035 | 59.971 | End: 5416 |
| Size | 20 | 20 | 128 |
| Primer 2 details |
|---|
| Sequence | ATGGGTGCCGCTATCGTTAG | CCGTCGGCTGCAAAAAGAAA | Start: 3661 |
| Tm | 59.966 | 59.97 | End: 3832 |
| Size | 20 | 20 | 172 |
| Primer 3 details |
|---|
| Sequence | ACGTTGACCAAGATGACCCC | GGTGATCTTGGAGTTGGGCA | Start: 7058 |
| Tm | 59.964 | 59.962 | End: 7297 |
| Size | 20 | 20 | 240 |
| Primer 4 details |
|---|
| Sequence | ACGTTGACCAAGATGACCCC | TGGTGATCTTGGAGTTGGGC | Start: 7058 |
| Tm | 59.964 | 59.962 | End: 7298 |
| Size | 20 | 20 | 241 |
| Primer 5 details |
|---|
| Sequence | ACCAGCATTATGCCCGACAA | CTGCACAAGACCACGAAAGC | Start: 5289 |
| Tm | 60.035 | 60.041 | End: 5421 |
| Size | 20 | 20 | 133 |
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