ssr_primer
Primer Details for TRINITY_DN13477_c0_g1_i1 |
|---|
| (T)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAAGAGGGATCCTACGGCA | TGCTGACTGGGGGAATTTCC | Start: 422 |
| Tm | 60.033 | 59.96 | End: 642 |
| Size | 20 | 20 | 221 |
| Primer 2 details |
|---|
| Sequence | GCTGCTTTCCGGGTTACTCT | GTCCCCACTCGCTTTCATCA | Start: 118 |
| Tm | 60.036 | 60.036 | End: 396 |
| Size | 20 | 20 | 279 |
| Primer 3 details |
|---|
| Sequence | AGCTGCTTTCCGGGTTACTC | GTCCCCACTCGCTTTCATCA | Start: 117 |
| Tm | 60.036 | 60.036 | End: 396 |
| Size | 20 | 20 | 280 |
| Primer 4 details |
|---|
| Sequence | TGATGAAAGCGAGTGGGGAC | TGCTGACTGGGGGAATTTCC | Start: 377 |
| Tm | 60.036 | 59.96 | End: 642 |
| Size | 20 | 20 | 266 |
| Primer 5 details |
|---|
| Sequence | TGAAGAGGGATCCTACGGCA | TTTTGGAGGTGCATTGGGGT | Start: 422 |
| Tm | 60.033 | 60.105 | End: 561 |
| Size | 20 | 20 | 140 |
(CT)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAAGAGGGATCCTACGGCA | TGCTGACTGGGGGAATTTCC | Start: 422 |
| Tm | 60.033 | 59.96 | End: 642 |
| Size | 20 | 20 | 221 |
| Primer 2 details |
|---|
| Sequence | GCTGCTTTCCGGGTTACTCT | GTCCCCACTCGCTTTCATCA | Start: 118 |
| Tm | 60.036 | 60.036 | End: 396 |
| Size | 20 | 20 | 279 |
| Primer 3 details |
|---|
| Sequence | AGCTGCTTTCCGGGTTACTC | GTCCCCACTCGCTTTCATCA | Start: 117 |
| Tm | 60.036 | 60.036 | End: 396 |
| Size | 20 | 20 | 280 |
| Primer 4 details |
|---|
| Sequence | TGATGAAAGCGAGTGGGGAC | TGCTGACTGGGGGAATTTCC | Start: 377 |
| Tm | 60.036 | 59.96 | End: 642 |
| Size | 20 | 20 | 266 |
| Primer 5 details |
|---|
| Sequence | TGAAGAGGGATCCTACGGCA | TTTTGGAGGTGCATTGGGGT | Start: 422 |
| Tm | 60.033 | 60.105 | End: 561 |
| Size | 20 | 20 | 140 |
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