ssr_primer
Primer Details for TRINITY_DN13475_c2_g1_i5 |
|---|
| (AT)7: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCATAGCTTTGACGCTTCGC | CCAATCGCTGACTTGGGTCT | Start: 3069 |
| Tm | 59.973 | 60.036 | End: 3334 |
| Size | 20 | 20 | 266 |
| Primer 2 details |
|---|
| Sequence | CCATAGCTTTGACGCTTCGC | TCCAATCGCTGACTTGGGTC | Start: 3069 |
| Tm | 59.973 | 60.036 | End: 3335 |
| Size | 20 | 20 | 267 |
| Primer 3 details |
|---|
| Sequence | ATGCTAGGCCCCATTCATCG | TGGACCCCACTGATAGGAGG | Start: 1704 |
| Tm | 59.963 | 60.031 | End: 1807 |
| Size | 20 | 20 | 104 |
| Primer 4 details |
|---|
| Sequence | ATGCTAGGCCCCATTCATCG | AATGGGAATTCCGTGGACCC | Start: 1704 |
| Tm | 59.963 | 60.034 | End: 1820 |
| Size | 20 | 20 | 117 |
| Primer 5 details |
|---|
| Sequence | CCTCCTATCAGTGGGGTCCA | TGGTTGCTTGCTGTGACTCA | Start: 1788 |
| Tm | 60.031 | 60.107 | End: 2036 |
| Size | 20 | 20 | 249 |
(AGA)5aaagaagaagaagggaaattgatcgaagaaatggagaaaaacagttgagaaaaaggaaagaagagagggg(GAAA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCATAGCTTTGACGCTTCGC | CCAATCGCTGACTTGGGTCT | Start: 3069 |
| Tm | 59.973 | 60.036 | End: 3334 |
| Size | 20 | 20 | 266 |
| Primer 2 details |
|---|
| Sequence | CCATAGCTTTGACGCTTCGC | TCCAATCGCTGACTTGGGTC | Start: 3069 |
| Tm | 59.973 | 60.036 | End: 3335 |
| Size | 20 | 20 | 267 |
| Primer 3 details |
|---|
| Sequence | ATGCTAGGCCCCATTCATCG | TGGACCCCACTGATAGGAGG | Start: 1704 |
| Tm | 59.963 | 60.031 | End: 1807 |
| Size | 20 | 20 | 104 |
| Primer 4 details |
|---|
| Sequence | ATGCTAGGCCCCATTCATCG | AATGGGAATTCCGTGGACCC | Start: 1704 |
| Tm | 59.963 | 60.034 | End: 1820 |
| Size | 20 | 20 | 117 |
| Primer 5 details |
|---|
| Sequence | CCTCCTATCAGTGGGGTCCA | TGGTTGCTTGCTGTGACTCA | Start: 1788 |
| Tm | 60.031 | 60.107 | End: 2036 |
| Size | 20 | 20 | 249 |
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