Primer Details for TRINITY_DN13474_c3_g1_i1 | |||
|---|---|---|---|
| (A)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCAATGTAATGAGTAATGGGGAGT | TGCTCCCACGTTTTTGTCCT | Start: 3 |
| Tm | 60.104 | 60.107 | End: 110 |
| Size | 25 | 20 | 108 |
| Primer 2 details | |||
| Sequence | GCAATGTAATGAGTAATGGGGAGT | TGCTCCCACGTTTTTGTCCT | Start: 4 |
| Tm | 58.867 | 60.107 | End: 110 |
| Size | 24 | 20 | 107 |
| Primer 3 details | |||
| Sequence | AGCAATGTAATGAGTAATGGGGAG | TGCTCCCACGTTTTTGTCCT | Start: 3 |
| Tm | 58.62 | 60.107 | End: 110 |
| Size | 24 | 20 | 108 |
| Primer 4 details | |||
| Sequence | AGCAATGTAATGAGTAATGGGGA | TGCTCCCACGTTTTTGTCCT | Start: 3 |
| Tm | 57.613 | 60.107 | End: 110 |
| Size | 23 | 20 | 108 |
| Primer 5 details | |||
| Sequence | GCAATGTAATGAGTAATGGGGAG | TGCTCCCACGTTTTTGTCCT | Start: 4 |
| Tm | 57.304 | 60.107 | End: 110 |
| Size | 23 | 20 | 107 |
