ssr_primer
Primer Details for TRINITY_DN13474_c1_g1_i6 |
|---|
| (T)20: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGATGTTTGCGTCCGAGGAA | TCACAGGGCATTCACTCCAC | Start: 414 |
| Tm | 59.966 | 59.963 | End: 665 |
| Size | 20 | 20 | 252 |
| Primer 2 details |
|---|
| Sequence | TTGATGTTTGCGTCCGAGGA | TCACAGGGCATTCACTCCAC | Start: 413 |
| Tm | 59.966 | 59.963 | End: 665 |
| Size | 20 | 20 | 253 |
| Primer 3 details |
|---|
| Sequence | TCGCAAGCGTGAGGTGTATT | GCGGTCAAAGGAGGAGTGAA | Start: 3402 |
| Tm | 60.038 | 59.965 | End: 3674 |
| Size | 20 | 20 | 273 |
| Primer 4 details |
|---|
| Sequence | TCCAACCATCTGGCCTTGTC | AATACACCTCACGCTTGCGA | Start: 3317 |
| Tm | 59.962 | 60.038 | End: 3421 |
| Size | 20 | 20 | 105 |
| Primer 5 details |
|---|
| Sequence | CTCCAACCATCTGGCCTTGT | AATACACCTCACGCTTGCGA | Start: 3316 |
| Tm | 59.961 | 60.038 | End: 3421 |
| Size | 20 | 20 | 106 |
(TTA)6atactactattacttgtacataagggggagcgtctcttggcagtcgcttctactcagtttttctcaattcttcttgttttcc(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGATGTTTGCGTCCGAGGAA | TCACAGGGCATTCACTCCAC | Start: 414 |
| Tm | 59.966 | 59.963 | End: 665 |
| Size | 20 | 20 | 252 |
| Primer 2 details |
|---|
| Sequence | TTGATGTTTGCGTCCGAGGA | TCACAGGGCATTCACTCCAC | Start: 413 |
| Tm | 59.966 | 59.963 | End: 665 |
| Size | 20 | 20 | 253 |
| Primer 3 details |
|---|
| Sequence | TCGCAAGCGTGAGGTGTATT | GCGGTCAAAGGAGGAGTGAA | Start: 3402 |
| Tm | 60.038 | 59.965 | End: 3674 |
| Size | 20 | 20 | 273 |
| Primer 4 details |
|---|
| Sequence | TCCAACCATCTGGCCTTGTC | AATACACCTCACGCTTGCGA | Start: 3317 |
| Tm | 59.962 | 60.038 | End: 3421 |
| Size | 20 | 20 | 105 |
| Primer 5 details |
|---|
| Sequence | CTCCAACCATCTGGCCTTGT | AATACACCTCACGCTTGCGA | Start: 3316 |
| Tm | 59.961 | 60.038 | End: 3421 |
| Size | 20 | 20 | 106 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGATGTTTGCGTCCGAGGAA | TCACAGGGCATTCACTCCAC | Start: 414 |
| Tm | 59.966 | 59.963 | End: 665 |
| Size | 20 | 20 | 252 |
| Primer 2 details |
|---|
| Sequence | TTGATGTTTGCGTCCGAGGA | TCACAGGGCATTCACTCCAC | Start: 413 |
| Tm | 59.966 | 59.963 | End: 665 |
| Size | 20 | 20 | 253 |
| Primer 3 details |
|---|
| Sequence | TCGCAAGCGTGAGGTGTATT | GCGGTCAAAGGAGGAGTGAA | Start: 3402 |
| Tm | 60.038 | 59.965 | End: 3674 |
| Size | 20 | 20 | 273 |
| Primer 4 details |
|---|
| Sequence | TCCAACCATCTGGCCTTGTC | AATACACCTCACGCTTGCGA | Start: 3317 |
| Tm | 59.962 | 60.038 | End: 3421 |
| Size | 20 | 20 | 105 |
| Primer 5 details |
|---|
| Sequence | CTCCAACCATCTGGCCTTGT | AATACACCTCACGCTTGCGA | Start: 3316 |
| Tm | 59.961 | 60.038 | End: 3421 |
| Size | 20 | 20 | 106 |
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