ssr_primer
Primer Details for TRINITY_DN13466_c0_g1_i4 |
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| (AT)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGCCGAAACTTGCAAGAGC | GAGGCAAACCAGAGGATGCT | Start: 6482 |
| Tm | 59.969 | 60.035 | End: 6649 |
| Size | 20 | 20 | 168 |
| Primer 2 details |
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| Sequence | GTGGCAGGGGAGAAGAAGTC | CTGCCAACATCTTCCTCCGT | Start: 5714 |
| Tm | 60.036 | 60.036 | End: 5921 |
| Size | 20 | 20 | 208 |
| Primer 3 details |
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| Sequence | GGTGGCACAGTCTGGATTGA | GACTTCTTCTCCCCTGCCAC | Start: 5602 |
| Tm | 59.963 | 60.036 | End: 5733 |
| Size | 20 | 20 | 132 |
| Primer 4 details |
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| Sequence | CTGGTGGCACAGTCTGGATT | GACTTCTTCTCCCCTGCCAC | Start: 5600 |
| Tm | 59.963 | 60.036 | End: 5733 |
| Size | 20 | 20 | 134 |
| Primer 5 details |
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| Sequence | TGTTCCAAGGGCATGAGACC | GACTTCTTCTCCCCTGCCAC | Start: 5488 |
| Tm | 59.962 | 60.036 | End: 5733 |
| Size | 20 | 20 | 246 |
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