ssr_primer
Primer Details for TRINITY_DN13465_c0_g1_i2 |
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| (A)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACAAGGGGGCCACCTAAAA | TTTCAGGGACATGAGGGTGC | Start: 670 |
| Tm | 60.179 | 59.962 | End: 773 |
| Size | 20 | 20 | 104 |
| Primer 2 details |
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| Sequence | CACAAGGGGGCCACCTAAAA | GTTGAACCGCAAACGTGTCA | Start: 670 |
| Tm | 60.179 | 59.903 | End: 848 |
| Size | 20 | 20 | 179 |
| Primer 3 details |
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| Sequence | CACAAGGGGGCCACCTAAAA | CGCAAACGTGTCAGTGGTTT | Start: 670 |
| Tm | 60.179 | 59.902 | End: 841 |
| Size | 20 | 20 | 172 |
| Primer 4 details |
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| Sequence | AATGTCCCAAACTGCACCCT | GTTGAACCGCAAACGTGTCA | Start: 741 |
| Tm | 59.812 | 59.903 | End: 848 |
| Size | 20 | 20 | 108 |
| Primer 5 details |
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| Sequence | AATGTCCCAAACTGCACCCT | CGCAAACGTGTCAGTGGTTT | Start: 741 |
| Tm | 59.812 | 59.902 | End: 841 |
| Size | 20 | 20 | 101 |
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