ssr_primer
Primer Details for TRINITY_DN13463_c6_g1_i8 |
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| (A)31: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGATCTACGCTGAAGACGCT | GAGGGTCGTGACTGCTCAAA | Start: 274 |
| Tm | 59.972 | 59.966 | End: 459 |
| Size | 20 | 20 | 186 |
| Primer 2 details |
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| Sequence | TCTTCGTTTGCGGAGAGCTT | AGCGTCTTCAGCGTAGATCG | Start: 167 |
| Tm | 59.966 | 59.972 | End: 293 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
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| Sequence | CGGTCTCCCTTAAAACCCCC | AGCGTCTTCAGCGTAGATCG | Start: 130 |
| Tm | 60.035 | 59.972 | End: 293 |
| Size | 20 | 20 | 164 |
| Primer 4 details |
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| Sequence | CCGGTCTCCCTTAAAACCCC | AGCGTCTTCAGCGTAGATCG | Start: 129 |
| Tm | 60.035 | 59.972 | End: 293 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
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| Sequence | GCTTCCCCTTCCAGTCCTTC | AGCGTCTTCAGCGTAGATCG | Start: 183 |
| Tm | 60.035 | 59.972 | End: 293 |
| Size | 20 | 20 | 111 |
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