ssr_primer
Primer Details for TRINITY_DN13463_c3_g1_i12 |
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| (T)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATCCGGCTGTTTCCTCCAG | GCGGATGCATTTCAGAACCC | Start: 3206 |
| Tm | 60.035 | 59.899 | End: 3428 |
| Size | 20 | 20 | 223 |
| Primer 2 details |
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| Sequence | AATCCGGCTGTTTCCTCCAG | GGCGGATGCATTTCAGAACC | Start: 3206 |
| Tm | 60.035 | 59.899 | End: 3429 |
| Size | 20 | 20 | 224 |
| Primer 3 details |
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| Sequence | AATCCGGCTGTTTCCTCCAG | CGCTGTTGGATTCCAAACCG | Start: 3206 |
| Tm | 60.035 | 60.11 | End: 3324 |
| Size | 20 | 20 | 119 |
| Primer 4 details |
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| Sequence | ATTCTGTGCAGCCAACCTCA | GCGCAGAAAAGTTTGGGGAG | Start: 2374 |
| Tm | 59.889 | 60.039 | End: 2519 |
| Size | 20 | 20 | 146 |
| Primer 5 details |
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| Sequence | AATCCGGCTGTTTCCTCCAG | CCTTTCCATTTCCCCACCCA | Start: 3206 |
| Tm | 60.035 | 59.884 | End: 3462 |
| Size | 20 | 20 | 257 |
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