ssr_primer
Primer Details for TRINITY_DN13463_c0_g1_i1 |
|---|
| (T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAGCAAAGGAGGAGATGGT | GGAGTTACAGTGACAGGCCC | Start: 454 |
| Tm | 60.035 | 60.036 | End: 684 |
| Size | 20 | 20 | 231 |
| Primer 2 details |
|---|
| Sequence | GGGCCTGTCACTGTAACTCC | CACTTCTGCCTCTCGCTCAA | Start: 665 |
| Tm | 60.036 | 60.038 | End: 938 |
| Size | 20 | 20 | 274 |
| Primer 3 details |
|---|
| Sequence | GGGCCTGTCACTGTAACTCC | TCCCCCTCCCAAAGTGAGAA | Start: 665 |
| Tm | 60.036 | 60.104 | End: 826 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
|---|
| Sequence | TTCTCACTTTGGGAGGGGGA | CACTTCTGCCTCTCGCTCAA | Start: 807 |
| Tm | 60.104 | 60.038 | End: 938 |
| Size | 20 | 20 | 132 |
| Primer 5 details |
|---|
| Sequence | GGGCCTGTCACTGTAACTCC | GGCCAGTAATTGTCTCCCCC | Start: 665 |
| Tm | 60.036 | 60.107 | End: 840 |
| Size | 20 | 20 | 176 |
(AT)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCAGCAAAGGAGGAGATGGT | GGAGTTACAGTGACAGGCCC | Start: 454 |
| Tm | 60.035 | 60.036 | End: 684 |
| Size | 20 | 20 | 231 |
| Primer 2 details |
|---|
| Sequence | GGGCCTGTCACTGTAACTCC | CACTTCTGCCTCTCGCTCAA | Start: 665 |
| Tm | 60.036 | 60.038 | End: 938 |
| Size | 20 | 20 | 274 |
| Primer 3 details |
|---|
| Sequence | GGGCCTGTCACTGTAACTCC | TCCCCCTCCCAAAGTGAGAA | Start: 665 |
| Tm | 60.036 | 60.104 | End: 826 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
|---|
| Sequence | TTCTCACTTTGGGAGGGGGA | CACTTCTGCCTCTCGCTCAA | Start: 807 |
| Tm | 60.104 | 60.038 | End: 938 |
| Size | 20 | 20 | 132 |
| Primer 5 details |
|---|
| Sequence | GGGCCTGTCACTGTAACTCC | GGCCAGTAATTGTCTCCCCC | Start: 665 |
| Tm | 60.036 | 60.107 | End: 840 |
| Size | 20 | 20 | 176 |
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