Primer Details for TRINITY_DN13451_c0_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCTACGAAGGTTTGTTTTTCT | ACAACCATAATACTTTCCCTCACGA | Start: 0 |
| Tm | 57.437 | 60.046 | End: 106 |
| Size | 22 | 25 | 107 |
| Primer 2 details |
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| Sequence | GGCTACGAAGGTTTGTTTTTCT | ACAACCATAATACTTTCCCTCACG | Start: 0 |
| Tm | 57.437 | 58.817 | End: 106 |
| Size | 22 | 24 | 107 |
| Primer 3 details |
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| Sequence | GGCTACGAAGGTTTGTTTTTCT | GACAACCATAATACTTTCCCTCACG | Start: 0 |
| Tm | 57.437 | 59.706 | End: 107 |
| Size | 22 | 25 | 108 |
| Primer 4 details |
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| Sequence | GGCTACGAAGGTTTGTTTTTCTT | ACAACCATAATACTTTCCCTCACGA | Start: 0 |
| Tm | 58.071 | 60.046 | End: 106 |
| Size | 23 | 25 | 107 |
| Primer 5 details |
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| Sequence | GGCTACGAAGGTTTGTTTTTCT | CAACCATAATACTTTCCCTCACGA | Start: 0 |
| Tm | 57.437 | 58.575 | End: 105 |
| Size | 22 | 24 | 106 |
