ssr_primer
Primer Details for TRINITY_DN13450_c1_g1_i4 |
|---|
| (T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCATCCCATCCTTGGCTTT | GCTGGTGCTGCATGTTTGAA | Start: 6589 |
| Tm | 60.032 | 59.968 | End: 6864 |
| Size | 20 | 20 | 276 |
| Primer 2 details |
|---|
| Sequence | CTTTTGAATCGGCTCCCCCT | TTCGAGTCCCTCAACTGTGC | Start: 3907 |
| Tm | 60.034 | 59.966 | End: 4076 |
| Size | 20 | 20 | 170 |
| Primer 3 details |
|---|
| Sequence | TAGCCACGTTCAAGGTGTCC | TTCGAGTCCCTCAACTGTGC | Start: 3800 |
| Tm | 59.966 | 59.966 | End: 4076 |
| Size | 20 | 20 | 277 |
| Primer 4 details |
|---|
| Sequence | TCCTTTTGAATCGGCTCCCC | TTCGAGTCCCTCAACTGTGC | Start: 3905 |
| Tm | 60.034 | 59.966 | End: 4076 |
| Size | 20 | 20 | 172 |
| Primer 5 details |
|---|
| Sequence | TTCAAACATGCAGCACCAGC | GACCCCCGTTGCTGATGTTA | Start: 6845 |
| Tm | 59.968 | 60.036 | End: 7018 |
| Size | 20 | 20 | 174 |
(AG)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCATCCCATCCTTGGCTTT | GCTGGTGCTGCATGTTTGAA | Start: 6589 |
| Tm | 60.032 | 59.968 | End: 6864 |
| Size | 20 | 20 | 276 |
| Primer 2 details |
|---|
| Sequence | CTTTTGAATCGGCTCCCCCT | TTCGAGTCCCTCAACTGTGC | Start: 3907 |
| Tm | 60.034 | 59.966 | End: 4076 |
| Size | 20 | 20 | 170 |
| Primer 3 details |
|---|
| Sequence | TAGCCACGTTCAAGGTGTCC | TTCGAGTCCCTCAACTGTGC | Start: 3800 |
| Tm | 59.966 | 59.966 | End: 4076 |
| Size | 20 | 20 | 277 |
| Primer 4 details |
|---|
| Sequence | TCCTTTTGAATCGGCTCCCC | TTCGAGTCCCTCAACTGTGC | Start: 3905 |
| Tm | 60.034 | 59.966 | End: 4076 |
| Size | 20 | 20 | 172 |
| Primer 5 details |
|---|
| Sequence | TTCAAACATGCAGCACCAGC | GACCCCCGTTGCTGATGTTA | Start: 6845 |
| Tm | 59.968 | 60.036 | End: 7018 |
| Size | 20 | 20 | 174 |
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