Primer Details for TRINITY_DN13449_c5_g1_i1 |
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| (A)61: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACATAGAAGTCTGCTGAATTTTTGT | ATTACAGATCGGGCCAGCTC | Start: 60 |
| Tm | 57.484 | 59.605 | End: 176 |
| Size | 25 | 20 | 117 |
| Primer 2 details |
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| Sequence | ACATAGAAGTCTGCTGAATTTTTGT | AGCTCCTCAGTCTGCCTCTA | Start: 60 |
| Tm | 57.484 | 59.371 | End: 161 |
| Size | 25 | 20 | 102 |
| Primer 3 details |
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| Sequence | ACATAGAAGTCTGCTGAATTTTTGTT | ATTACAGATCGGGCCAGCTC | Start: 60 |
| Tm | 58.055 | 59.605 | End: 176 |
| Size | 26 | 20 | 117 |
| Primer 4 details |
|---|
| Sequence | AACATAGAAGTCTGCTGAATTTTTGT | ATTACAGATCGGGCCAGCTC | Start: 59 |
| Tm | 58.055 | 59.605 | End: 176 |
| Size | 26 | 20 | 118 |
| Primer 5 details |
|---|
| Sequence | ACATAGAAGTCTGCTGAATTTTTGT | AATTACAGATCGGGCCAGCT | Start: 60 |
| Tm | 57.484 | 59.16 | End: 177 |
| Size | 25 | 20 | 118 |
(A)23: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACATAGAAGTCTGCTGAATTTTTGT | ATTACAGATCGGGCCAGCTC | Start: 60 |
| Tm | 57.484 | 59.605 | End: 176 |
| Size | 25 | 20 | 117 |
| Primer 2 details |
|---|
| Sequence | ACATAGAAGTCTGCTGAATTTTTGT | AGCTCCTCAGTCTGCCTCTA | Start: 60 |
| Tm | 57.484 | 59.371 | End: 161 |
| Size | 25 | 20 | 102 |
| Primer 3 details |
|---|
| Sequence | ACATAGAAGTCTGCTGAATTTTTGTT | ATTACAGATCGGGCCAGCTC | Start: 60 |
| Tm | 58.055 | 59.605 | End: 176 |
| Size | 26 | 20 | 117 |
| Primer 4 details |
|---|
| Sequence | AACATAGAAGTCTGCTGAATTTTTGT | ATTACAGATCGGGCCAGCTC | Start: 59 |
| Tm | 58.055 | 59.605 | End: 176 |
| Size | 26 | 20 | 118 |
| Primer 5 details |
|---|
| Sequence | ACATAGAAGTCTGCTGAATTTTTGT | AATTACAGATCGGGCCAGCT | Start: 60 |
| Tm | 57.484 | 59.16 | End: 177 |
| Size | 25 | 20 | 118 |
