ssr_primer
Primer Details for TRINITY_DN13447_c1_g1_i10 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCTCCACCGATTCAGGGGA | CATTGCTCCCAACCCCTGAT | Start: 471 |
| Tm | 60.031 | 60.033 | End: 743 |
| Size | 20 | 20 | 273 |
| Primer 2 details |
|---|
| Sequence | TGTCTTACACACCGGACACG | GCTTGAACTCGGGGAAAGGA | Start: 3462 |
| Tm | 59.969 | 59.964 | End: 3726 |
| Size | 20 | 20 | 265 |
| Primer 3 details |
|---|
| Sequence | GTCTTACACACCGGACACGT | GCTTGAACTCGGGGAAAGGA | Start: 3463 |
| Tm | 59.969 | 59.964 | End: 3726 |
| Size | 20 | 20 | 264 |
| Primer 4 details |
|---|
| Sequence | CAGGGGACAAGGGCACTTAG | CATTGCTCCCAACCCCTGAT | Start: 484 |
| Tm | 60.035 | 60.033 | End: 743 |
| Size | 20 | 20 | 260 |
| Primer 5 details |
|---|
| Sequence | GCTGTGCCACAATGAAAGCA | TCCCCAAGAATTTCCGTCGG | Start: 3043 |
| Tm | 59.968 | 60.036 | End: 3181 |
| Size | 20 | 20 | 139 |
(GAA)9: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCTCCACCGATTCAGGGGA | CATTGCTCCCAACCCCTGAT | Start: 471 |
| Tm | 60.031 | 60.033 | End: 743 |
| Size | 20 | 20 | 273 |
| Primer 2 details |
|---|
| Sequence | TGTCTTACACACCGGACACG | GCTTGAACTCGGGGAAAGGA | Start: 3462 |
| Tm | 59.969 | 59.964 | End: 3726 |
| Size | 20 | 20 | 265 |
| Primer 3 details |
|---|
| Sequence | GTCTTACACACCGGACACGT | GCTTGAACTCGGGGAAAGGA | Start: 3463 |
| Tm | 59.969 | 59.964 | End: 3726 |
| Size | 20 | 20 | 264 |
| Primer 4 details |
|---|
| Sequence | CAGGGGACAAGGGCACTTAG | CATTGCTCCCAACCCCTGAT | Start: 484 |
| Tm | 60.035 | 60.033 | End: 743 |
| Size | 20 | 20 | 260 |
| Primer 5 details |
|---|
| Sequence | GCTGTGCCACAATGAAAGCA | TCCCCAAGAATTTCCGTCGG | Start: 3043 |
| Tm | 59.968 | 60.036 | End: 3181 |
| Size | 20 | 20 | 139 |
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