ssr_primer
Primer Details for TRINITY_DN13442_c0_g2_i7 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGACACGTGAAAACGCAGC | CTGCTGACTTCCCACTTCGT | Start: 2369 |
| Tm | 59.972 | 59.966 | End: 2517 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
|---|
| Sequence | AAGACACGTGAAAACGCAGC | ACTGCTGACTTCCCACTTCG | Start: 2369 |
| Tm | 59.972 | 59.966 | End: 2518 |
| Size | 20 | 20 | 150 |
| Primer 3 details |
|---|
| Sequence | ATCCCCTGAAGACTCGACGA | TGCCTTTTGGGTTCAAGGGT | Start: 864 |
| Tm | 60.034 | 60.032 | End: 1134 |
| Size | 20 | 20 | 271 |
| Primer 4 details |
|---|
| Sequence | GTTTCCCCTCTGCTTCTCCC | GGAATTGGTCCCGTCGAAGT | Start: 2804 |
| Tm | 60.035 | 60.037 | End: 2918 |
| Size | 20 | 20 | 115 |
| Primer 5 details |
|---|
| Sequence | CCATCTCAAGCACACCCAGT | CCATCTGGTTCACTGCACCT | Start: 344 |
| Tm | 59.963 | 59.963 | End: 484 |
| Size | 20 | 20 | 141 |
(T)10gtaacctacatacattcagggtagggaatgtaaataaaaatgattgaaaatcattcagctgtagaaaccaatgtttatacac(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGACACGTGAAAACGCAGC | CTGCTGACTTCCCACTTCGT | Start: 2369 |
| Tm | 59.972 | 59.966 | End: 2517 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
|---|
| Sequence | AAGACACGTGAAAACGCAGC | ACTGCTGACTTCCCACTTCG | Start: 2369 |
| Tm | 59.972 | 59.966 | End: 2518 |
| Size | 20 | 20 | 150 |
| Primer 3 details |
|---|
| Sequence | ATCCCCTGAAGACTCGACGA | TGCCTTTTGGGTTCAAGGGT | Start: 864 |
| Tm | 60.034 | 60.032 | End: 1134 |
| Size | 20 | 20 | 271 |
| Primer 4 details |
|---|
| Sequence | GTTTCCCCTCTGCTTCTCCC | GGAATTGGTCCCGTCGAAGT | Start: 2804 |
| Tm | 60.035 | 60.037 | End: 2918 |
| Size | 20 | 20 | 115 |
| Primer 5 details |
|---|
| Sequence | CCATCTCAAGCACACCCAGT | CCATCTGGTTCACTGCACCT | Start: 344 |
| Tm | 59.963 | 59.963 | End: 484 |
| Size | 20 | 20 | 141 |
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