ssr_primer
Primer Details for TRINITY_DN13431_c3_g1_i5 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGGGGCAGGATCTGAATCG | ACATGTAAGCTGGGACACCG | Start: 5014 |
| Tm | 59.965 | 60.036 | End: 5173 |
| Size | 20 | 20 | 160 |
| Primer 2 details |
|---|
| Sequence | CGTACCTTGGATGCCTGGTT | ACCAGTTGGCCATGTCTCTG | Start: 2250 |
| Tm | 60.035 | 59.963 | End: 2509 |
| Size | 20 | 20 | 260 |
| Primer 3 details |
|---|
| Sequence | GGATCTGAATCGTGCGGTCT | ACATGTAAGCTGGGACACCG | Start: 5022 |
| Tm | 59.897 | 60.036 | End: 5173 |
| Size | 20 | 20 | 152 |
| Primer 4 details |
|---|
| Sequence | AGGATCTGAATCGTGCGGTC | ACATGTAAGCTGGGACACCG | Start: 5021 |
| Tm | 59.897 | 60.036 | End: 5173 |
| Size | 20 | 20 | 153 |
| Primer 5 details |
|---|
| Sequence | GAGCTATGGGGACGTCCAAG | ACGGCCAGAAACGGAAGAAT | Start: 214 |
| Tm | 59.895 | 59.964 | End: 364 |
| Size | 20 | 20 | 151 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGGGGCAGGATCTGAATCG | ACATGTAAGCTGGGACACCG | Start: 5014 |
| Tm | 59.965 | 60.036 | End: 5173 |
| Size | 20 | 20 | 160 |
| Primer 2 details |
|---|
| Sequence | CGTACCTTGGATGCCTGGTT | ACCAGTTGGCCATGTCTCTG | Start: 2250 |
| Tm | 60.035 | 59.963 | End: 2509 |
| Size | 20 | 20 | 260 |
| Primer 3 details |
|---|
| Sequence | GGATCTGAATCGTGCGGTCT | ACATGTAAGCTGGGACACCG | Start: 5022 |
| Tm | 59.897 | 60.036 | End: 5173 |
| Size | 20 | 20 | 152 |
| Primer 4 details |
|---|
| Sequence | AGGATCTGAATCGTGCGGTC | ACATGTAAGCTGGGACACCG | Start: 5021 |
| Tm | 59.897 | 60.036 | End: 5173 |
| Size | 20 | 20 | 153 |
| Primer 5 details |
|---|
| Sequence | GAGCTATGGGGACGTCCAAG | ACGGCCAGAAACGGAAGAAT | Start: 214 |
| Tm | 59.895 | 59.964 | End: 364 |
| Size | 20 | 20 | 151 |
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