ssr_primer
Primer Details for TRINITY_DN13411_c2_g1_i9 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCGTTTGGTTTTTCGACGC | ACAAACGACGGAAGCAAACG | Start: 3854 |
| Tm | 59.972 | 59.973 | End: 3959 |
| Size | 20 | 20 | 106 |
| Primer 2 details |
|---|
| Sequence | CCCGGGAGAATATGCTCGAC | GGCGAAAATTCCGATGGAGC | Start: 2839 |
| Tm | 60.038 | 59.971 | End: 3099 |
| Size | 20 | 20 | 261 |
| Primer 3 details |
|---|
| Sequence | CCCGGGAGAATATGCTCGAC | CTCAACCTCGTCCGTTTGGA | Start: 2839 |
| Tm | 60.038 | 59.967 | End: 3076 |
| Size | 20 | 20 | 238 |
| Primer 4 details |
|---|
| Sequence | CCCGGGAGAATATGCTCGAC | TCCTCAACCTCGTCCGTTTG | Start: 2839 |
| Tm | 60.038 | 59.967 | End: 3078 |
| Size | 20 | 20 | 240 |
| Primer 5 details |
|---|
| Sequence | TGCTCGCTTGCCAAGTTCTA | GCTGTCGCAAGTGAAATGCA | Start: 1447 |
| Tm | 59.965 | 60.04 | End: 1715 |
| Size | 20 | 20 | 269 |
(AG)11gagaagaagtttagatttaatgttacatac(AT)7(TA)8: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCGTTTGGTTTTTCGACGC | ACAAACGACGGAAGCAAACG | Start: 3854 |
| Tm | 59.972 | 59.973 | End: 3959 |
| Size | 20 | 20 | 106 |
| Primer 2 details |
|---|
| Sequence | CCCGGGAGAATATGCTCGAC | GGCGAAAATTCCGATGGAGC | Start: 2839 |
| Tm | 60.038 | 59.971 | End: 3099 |
| Size | 20 | 20 | 261 |
| Primer 3 details |
|---|
| Sequence | CCCGGGAGAATATGCTCGAC | CTCAACCTCGTCCGTTTGGA | Start: 2839 |
| Tm | 60.038 | 59.967 | End: 3076 |
| Size | 20 | 20 | 238 |
| Primer 4 details |
|---|
| Sequence | CCCGGGAGAATATGCTCGAC | TCCTCAACCTCGTCCGTTTG | Start: 2839 |
| Tm | 60.038 | 59.967 | End: 3078 |
| Size | 20 | 20 | 240 |
| Primer 5 details |
|---|
| Sequence | TGCTCGCTTGCCAAGTTCTA | GCTGTCGCAAGTGAAATGCA | Start: 1447 |
| Tm | 59.965 | 60.04 | End: 1715 |
| Size | 20 | 20 | 269 |
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