Primer Details for TRINITY_DN13380_c0_g2_i1 | |||
|---|---|---|---|
| (A)15ttaaacaaaaag(A)17: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGGAAAACGACCAAAACAAACA | CGCTAGTCTTTAGATCCAAATGGT | Start: 82 |
| Tm | 59.993 | 58.639 | End: 258 |
| Size | 23 | 24 | 177 |
| Primer 2 details | |||
| Sequence | TGATCTTTGGGAAAACGACCA | CGCTAGTCTTTAGATCCAAATGGT | Start: 75 |
| Tm | 57.784 | 58.639 | End: 258 |
| Size | 21 | 24 | 184 |
| Primer 3 details | |||
| Sequence | TGGGAAAACGACCAAAACAAACA | CGCTAGTCTTTAGATCCAAATGGTT | Start: 82 |
| Tm | 59.993 | 59.182 | End: 258 |
| Size | 23 | 25 | 177 |
| Primer 4 details | |||
| Sequence | TGATCTTTGGGAAAACGACCAA | CGCTAGTCTTTAGATCCAAATGGT | Start: 75 |
| Tm | 58.443 | 58.639 | End: 258 |
| Size | 22 | 24 | 184 |
| Primer 5 details | |||
| Sequence | TGGGAAAACGACCAAAACAAACA | CGCTAGTCTTTAGATCCAAATGG | Start: 82 |
| Tm | 59.993 | 57.086 | End: 258 |
| Size | 23 | 23 | 177 |
