ssr_primer
Primer Details for TRINITY_DN13378_c0_g1_i3 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCATGGGAAGAGGGTGACAA | GGGCTCACATGGACCTCAAA | Start: 1419 |
| Tm | 59.962 | 59.962 | End: 1692 |
| Size | 20 | 20 | 274 |
| Primer 2 details |
|---|
| Sequence | CTGCAGGAGAATGCCAATGC | CCATGCCCACAAAACATGCA | Start: 1215 |
| Tm | 59.898 | 59.965 | End: 1424 |
| Size | 20 | 20 | 210 |
| Primer 3 details |
|---|
| Sequence | CTGCAGGAGAATGCCAATGC | TTGTCACCCTCTTCCCATGC | Start: 1215 |
| Tm | 59.898 | 59.962 | End: 1438 |
| Size | 20 | 20 | 224 |
| Primer 4 details |
|---|
| Sequence | AATGAAGGCGCGAGAGAGAG | TCGCAGCAACAATTGGAAGC | Start: 257 |
| Tm | 59.898 | 60.039 | End: 412 |
| Size | 20 | 20 | 156 |
| Primer 5 details |
|---|
| Sequence | GCGCGAGAGAGAGAAAAGGA | TCGCAGCAACAATTGGAAGC | Start: 264 |
| Tm | 59.829 | 60.039 | End: 412 |
| Size | 20 | 20 | 149 |
(T)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCATGGGAAGAGGGTGACAA | GGGCTCACATGGACCTCAAA | Start: 1419 |
| Tm | 59.962 | 59.962 | End: 1692 |
| Size | 20 | 20 | 274 |
| Primer 2 details |
|---|
| Sequence | CTGCAGGAGAATGCCAATGC | CCATGCCCACAAAACATGCA | Start: 1215 |
| Tm | 59.898 | 59.965 | End: 1424 |
| Size | 20 | 20 | 210 |
| Primer 3 details |
|---|
| Sequence | CTGCAGGAGAATGCCAATGC | TTGTCACCCTCTTCCCATGC | Start: 1215 |
| Tm | 59.898 | 59.962 | End: 1438 |
| Size | 20 | 20 | 224 |
| Primer 4 details |
|---|
| Sequence | AATGAAGGCGCGAGAGAGAG | TCGCAGCAACAATTGGAAGC | Start: 257 |
| Tm | 59.898 | 60.039 | End: 412 |
| Size | 20 | 20 | 156 |
| Primer 5 details |
|---|
| Sequence | GCGCGAGAGAGAGAAAAGGA | TCGCAGCAACAATTGGAAGC | Start: 264 |
| Tm | 59.829 | 60.039 | End: 412 |
| Size | 20 | 20 | 149 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCATGGGAAGAGGGTGACAA | GGGCTCACATGGACCTCAAA | Start: 1419 |
| Tm | 59.962 | 59.962 | End: 1692 |
| Size | 20 | 20 | 274 |
| Primer 2 details |
|---|
| Sequence | CTGCAGGAGAATGCCAATGC | CCATGCCCACAAAACATGCA | Start: 1215 |
| Tm | 59.898 | 59.965 | End: 1424 |
| Size | 20 | 20 | 210 |
| Primer 3 details |
|---|
| Sequence | CTGCAGGAGAATGCCAATGC | TTGTCACCCTCTTCCCATGC | Start: 1215 |
| Tm | 59.898 | 59.962 | End: 1438 |
| Size | 20 | 20 | 224 |
| Primer 4 details |
|---|
| Sequence | AATGAAGGCGCGAGAGAGAG | TCGCAGCAACAATTGGAAGC | Start: 257 |
| Tm | 59.898 | 60.039 | End: 412 |
| Size | 20 | 20 | 156 |
| Primer 5 details |
|---|
| Sequence | GCGCGAGAGAGAGAAAAGGA | TCGCAGCAACAATTGGAAGC | Start: 264 |
| Tm | 59.829 | 60.039 | End: 412 |
| Size | 20 | 20 | 149 |
(AT)10gtcaaattcaagtttccaacttttgtatttatatcattttattgtaataatttcattttttttcg(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCATGGGAAGAGGGTGACAA | GGGCTCACATGGACCTCAAA | Start: 1419 |
| Tm | 59.962 | 59.962 | End: 1692 |
| Size | 20 | 20 | 274 |
| Primer 2 details |
|---|
| Sequence | CTGCAGGAGAATGCCAATGC | CCATGCCCACAAAACATGCA | Start: 1215 |
| Tm | 59.898 | 59.965 | End: 1424 |
| Size | 20 | 20 | 210 |
| Primer 3 details |
|---|
| Sequence | CTGCAGGAGAATGCCAATGC | TTGTCACCCTCTTCCCATGC | Start: 1215 |
| Tm | 59.898 | 59.962 | End: 1438 |
| Size | 20 | 20 | 224 |
| Primer 4 details |
|---|
| Sequence | AATGAAGGCGCGAGAGAGAG | TCGCAGCAACAATTGGAAGC | Start: 257 |
| Tm | 59.898 | 60.039 | End: 412 |
| Size | 20 | 20 | 156 |
| Primer 5 details |
|---|
| Sequence | GCGCGAGAGAGAGAAAAGGA | TCGCAGCAACAATTGGAAGC | Start: 264 |
| Tm | 59.829 | 60.039 | End: 412 |
| Size | 20 | 20 | 149 |
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