ssr_primer
Primer Details for TRINITY_DN13361_c0_g4_i2 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTGGGTTCAATGCCTCCCT | GTCGATCTGGTAGTCGCAGG | Start: 1121 |
| Tm | 59.96 | 59.97 | End: 1308 |
| Size | 20 | 20 | 188 |
| Primer 2 details |
|---|
| Sequence | AACCGTATCATCCTCGACGC | AGGGAGGCATTGAACCCAAG | Start: 915 |
| Tm | 59.97 | 59.96 | End: 1140 |
| Size | 20 | 20 | 226 |
| Primer 3 details |
|---|
| Sequence | GGCGATGGATCTTACTCCCG | TTTGAGAAATCGGCTCCCCC | Start: 1312 |
| Tm | 60.038 | 60.034 | End: 1577 |
| Size | 20 | 20 | 266 |
| Primer 4 details |
|---|
| Sequence | CGGCGATGGATCTTACTCCC | TTTGAGAAATCGGCTCCCCC | Start: 1311 |
| Tm | 60.038 | 60.034 | End: 1577 |
| Size | 20 | 20 | 267 |
| Primer 5 details |
|---|
| Sequence | GCCAGACTCACCAACTCTCC | AGGGAGGCATTGAACCCAAG | Start: 986 |
| Tm | 60.037 | 59.96 | End: 1140 |
| Size | 20 | 20 | 155 |
(AT)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTGGGTTCAATGCCTCCCT | GTCGATCTGGTAGTCGCAGG | Start: 1121 |
| Tm | 59.96 | 59.97 | End: 1308 |
| Size | 20 | 20 | 188 |
| Primer 2 details |
|---|
| Sequence | AACCGTATCATCCTCGACGC | AGGGAGGCATTGAACCCAAG | Start: 915 |
| Tm | 59.97 | 59.96 | End: 1140 |
| Size | 20 | 20 | 226 |
| Primer 3 details |
|---|
| Sequence | GGCGATGGATCTTACTCCCG | TTTGAGAAATCGGCTCCCCC | Start: 1312 |
| Tm | 60.038 | 60.034 | End: 1577 |
| Size | 20 | 20 | 266 |
| Primer 4 details |
|---|
| Sequence | CGGCGATGGATCTTACTCCC | TTTGAGAAATCGGCTCCCCC | Start: 1311 |
| Tm | 60.038 | 60.034 | End: 1577 |
| Size | 20 | 20 | 267 |
| Primer 5 details |
|---|
| Sequence | GCCAGACTCACCAACTCTCC | AGGGAGGCATTGAACCCAAG | Start: 986 |
| Tm | 60.037 | 59.96 | End: 1140 |
| Size | 20 | 20 | 155 |
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