ssr_primer
Primer Details for TRINITY_DN13355_c3_g1_i4 |
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| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGGGGAACAATTCTCGGCT | TTCGACCTAAGCACCACACC | Start: 190 |
| Tm | 60.035 | 59.966 | End: 359 |
| Size | 20 | 20 | 170 |
| Primer 2 details |
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| Sequence | GCCGAAGATGGTGAAGGGAA | AGCCGAGAATTGTTCCCCTG | Start: 35 |
| Tm | 60.035 | 60.035 | End: 209 |
| Size | 20 | 20 | 175 |
| Primer 3 details |
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| Sequence | GGTGTGGTGCTTAGGTCGAA | AGGTCTTGCTACTTTGCCCC | Start: 340 |
| Tm | 59.966 | 59.962 | End: 522 |
| Size | 20 | 20 | 183 |
| Primer 4 details |
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| Sequence | ATGGTGAAGGGAAGGCAAGG | AGCCGAGAATTGTTCCCCTG | Start: 42 |
| Tm | 59.96 | 60.035 | End: 209 |
| Size | 20 | 20 | 168 |
| Primer 5 details |
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| Sequence | CCAGACTCTACACCAGGGGA | TTCGACCTAAGCACCACACC | Start: 177 |
| Tm | 59.959 | 59.966 | End: 359 |
| Size | 20 | 20 | 183 |
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