ssr_primer
Primer Details for TRINITY_DN13352_c2_g2_i1 |
|---|
| (A)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTGCTCCCCGTATCTTCA | TGGTGGACGATGCACAATCA | Start: 955 |
| Tm | 60.033 | 59.964 | End: 1092 |
| Size | 20 | 20 | 138 |
| Primer 2 details |
|---|
| Sequence | CGTGGGAGGAGAGTGCAATT | TCCACGTCTCAGGAGGGATT | Start: 429 |
| Tm | 60.036 | 59.959 | End: 577 |
| Size | 20 | 20 | 149 |
| Primer 3 details |
|---|
| Sequence | TCCTGCTCCCCGTATCTTCA | AATCGAGGATCCAACGACGG | Start: 955 |
| Tm | 60.033 | 59.898 | End: 1115 |
| Size | 20 | 20 | 161 |
| Primer 4 details |
|---|
| Sequence | CTGCTCCCCGTATCTTCACC | TGGTGGACGATGCACAATCA | Start: 957 |
| Tm | 59.895 | 59.964 | End: 1092 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
|---|
| Sequence | CCTGCTCCCCGTATCTTCAC | TGGTGGACGATGCACAATCA | Start: 956 |
| Tm | 59.895 | 59.964 | End: 1092 |
| Size | 20 | 20 | 137 |
(T)12ctgttcatttgaatgaaataatattttatatgataaataaataaaaagaaatag(AGAA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTGCTCCCCGTATCTTCA | TGGTGGACGATGCACAATCA | Start: 955 |
| Tm | 60.033 | 59.964 | End: 1092 |
| Size | 20 | 20 | 138 |
| Primer 2 details |
|---|
| Sequence | CGTGGGAGGAGAGTGCAATT | TCCACGTCTCAGGAGGGATT | Start: 429 |
| Tm | 60.036 | 59.959 | End: 577 |
| Size | 20 | 20 | 149 |
| Primer 3 details |
|---|
| Sequence | TCCTGCTCCCCGTATCTTCA | AATCGAGGATCCAACGACGG | Start: 955 |
| Tm | 60.033 | 59.898 | End: 1115 |
| Size | 20 | 20 | 161 |
| Primer 4 details |
|---|
| Sequence | CTGCTCCCCGTATCTTCACC | TGGTGGACGATGCACAATCA | Start: 957 |
| Tm | 59.895 | 59.964 | End: 1092 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
|---|
| Sequence | CCTGCTCCCCGTATCTTCAC | TGGTGGACGATGCACAATCA | Start: 956 |
| Tm | 59.895 | 59.964 | End: 1092 |
| Size | 20 | 20 | 137 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTGCTCCCCGTATCTTCA | TGGTGGACGATGCACAATCA | Start: 955 |
| Tm | 60.033 | 59.964 | End: 1092 |
| Size | 20 | 20 | 138 |
| Primer 2 details |
|---|
| Sequence | CGTGGGAGGAGAGTGCAATT | TCCACGTCTCAGGAGGGATT | Start: 429 |
| Tm | 60.036 | 59.959 | End: 577 |
| Size | 20 | 20 | 149 |
| Primer 3 details |
|---|
| Sequence | TCCTGCTCCCCGTATCTTCA | AATCGAGGATCCAACGACGG | Start: 955 |
| Tm | 60.033 | 59.898 | End: 1115 |
| Size | 20 | 20 | 161 |
| Primer 4 details |
|---|
| Sequence | CTGCTCCCCGTATCTTCACC | TGGTGGACGATGCACAATCA | Start: 957 |
| Tm | 59.895 | 59.964 | End: 1092 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
|---|
| Sequence | CCTGCTCCCCGTATCTTCAC | TGGTGGACGATGCACAATCA | Start: 956 |
| Tm | 59.895 | 59.964 | End: 1092 |
| Size | 20 | 20 | 137 |
(GA)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTGCTCCCCGTATCTTCA | TGGTGGACGATGCACAATCA | Start: 955 |
| Tm | 60.033 | 59.964 | End: 1092 |
| Size | 20 | 20 | 138 |
| Primer 2 details |
|---|
| Sequence | CGTGGGAGGAGAGTGCAATT | TCCACGTCTCAGGAGGGATT | Start: 429 |
| Tm | 60.036 | 59.959 | End: 577 |
| Size | 20 | 20 | 149 |
| Primer 3 details |
|---|
| Sequence | TCCTGCTCCCCGTATCTTCA | AATCGAGGATCCAACGACGG | Start: 955 |
| Tm | 60.033 | 59.898 | End: 1115 |
| Size | 20 | 20 | 161 |
| Primer 4 details |
|---|
| Sequence | CTGCTCCCCGTATCTTCACC | TGGTGGACGATGCACAATCA | Start: 957 |
| Tm | 59.895 | 59.964 | End: 1092 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
|---|
| Sequence | CCTGCTCCCCGTATCTTCAC | TGGTGGACGATGCACAATCA | Start: 956 |
| Tm | 59.895 | 59.964 | End: 1092 |
| Size | 20 | 20 | 137 |
(A)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTGCTCCCCGTATCTTCA | TGGTGGACGATGCACAATCA | Start: 955 |
| Tm | 60.033 | 59.964 | End: 1092 |
| Size | 20 | 20 | 138 |
| Primer 2 details |
|---|
| Sequence | CGTGGGAGGAGAGTGCAATT | TCCACGTCTCAGGAGGGATT | Start: 429 |
| Tm | 60.036 | 59.959 | End: 577 |
| Size | 20 | 20 | 149 |
| Primer 3 details |
|---|
| Sequence | TCCTGCTCCCCGTATCTTCA | AATCGAGGATCCAACGACGG | Start: 955 |
| Tm | 60.033 | 59.898 | End: 1115 |
| Size | 20 | 20 | 161 |
| Primer 4 details |
|---|
| Sequence | CTGCTCCCCGTATCTTCACC | TGGTGGACGATGCACAATCA | Start: 957 |
| Tm | 59.895 | 59.964 | End: 1092 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
|---|
| Sequence | CCTGCTCCCCGTATCTTCAC | TGGTGGACGATGCACAATCA | Start: 956 |
| Tm | 59.895 | 59.964 | End: 1092 |
| Size | 20 | 20 | 137 |
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