ssr_primer
Primer Details for TRINITY_DN13352_c2_g1_i3 |
|---|
| (TCT)5tcatcatcttccactcctcttccctctcttctccatctcttcctttcattctt(TC)6tacacaccatgaaatatccattcccatactgataattcccccta(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTTCACCGGAATCAACGA | ACAGAGGCGCCAGAAATTGA | Start: 286 |
| Tm | 59.966 | 59.963 | End: 467 |
| Size | 20 | 20 | 182 |
| Primer 2 details |
|---|
| Sequence | GGAGTGAGATGCAAGTGGCT | TCCTCAAGTGGCAGCAGATG | Start: 3707 |
| Tm | 60.036 | 60.036 | End: 3826 |
| Size | 20 | 20 | 120 |
| Primer 3 details |
|---|
| Sequence | TGCTTCACCGGAATCAACGA | CACTTACAGAGGCGCCAGAA | Start: 286 |
| Tm | 59.966 | 60.037 | End: 472 |
| Size | 20 | 20 | 187 |
| Primer 4 details |
|---|
| Sequence | GGAGGATGGGGCACAAGAAA | AGACTTATCGGCTGGGGAGT | Start: 4299 |
| Tm | 59.96 | 60.032 | End: 4483 |
| Size | 20 | 20 | 185 |
| Primer 5 details |
|---|
| Sequence | GCTCGAGTCTTCCACGTCAA | GGTCCAGCAGAGTTTCGTGA | Start: 4859 |
| Tm | 60.039 | 59.966 | End: 5043 |
| Size | 20 | 20 | 185 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTTCACCGGAATCAACGA | ACAGAGGCGCCAGAAATTGA | Start: 286 |
| Tm | 59.966 | 59.963 | End: 467 |
| Size | 20 | 20 | 182 |
| Primer 2 details |
|---|
| Sequence | GGAGTGAGATGCAAGTGGCT | TCCTCAAGTGGCAGCAGATG | Start: 3707 |
| Tm | 60.036 | 60.036 | End: 3826 |
| Size | 20 | 20 | 120 |
| Primer 3 details |
|---|
| Sequence | TGCTTCACCGGAATCAACGA | CACTTACAGAGGCGCCAGAA | Start: 286 |
| Tm | 59.966 | 60.037 | End: 472 |
| Size | 20 | 20 | 187 |
| Primer 4 details |
|---|
| Sequence | GGAGGATGGGGCACAAGAAA | AGACTTATCGGCTGGGGAGT | Start: 4299 |
| Tm | 59.96 | 60.032 | End: 4483 |
| Size | 20 | 20 | 185 |
| Primer 5 details |
|---|
| Sequence | GCTCGAGTCTTCCACGTCAA | GGTCCAGCAGAGTTTCGTGA | Start: 4859 |
| Tm | 60.039 | 59.966 | End: 5043 |
| Size | 20 | 20 | 185 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTTCACCGGAATCAACGA | ACAGAGGCGCCAGAAATTGA | Start: 286 |
| Tm | 59.966 | 59.963 | End: 467 |
| Size | 20 | 20 | 182 |
| Primer 2 details |
|---|
| Sequence | GGAGTGAGATGCAAGTGGCT | TCCTCAAGTGGCAGCAGATG | Start: 3707 |
| Tm | 60.036 | 60.036 | End: 3826 |
| Size | 20 | 20 | 120 |
| Primer 3 details |
|---|
| Sequence | TGCTTCACCGGAATCAACGA | CACTTACAGAGGCGCCAGAA | Start: 286 |
| Tm | 59.966 | 60.037 | End: 472 |
| Size | 20 | 20 | 187 |
| Primer 4 details |
|---|
| Sequence | GGAGGATGGGGCACAAGAAA | AGACTTATCGGCTGGGGAGT | Start: 4299 |
| Tm | 59.96 | 60.032 | End: 4483 |
| Size | 20 | 20 | 185 |
| Primer 5 details |
|---|
| Sequence | GCTCGAGTCTTCCACGTCAA | GGTCCAGCAGAGTTTCGTGA | Start: 4859 |
| Tm | 60.039 | 59.966 | End: 5043 |
| Size | 20 | 20 | 185 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTTCACCGGAATCAACGA | ACAGAGGCGCCAGAAATTGA | Start: 286 |
| Tm | 59.966 | 59.963 | End: 467 |
| Size | 20 | 20 | 182 |
| Primer 2 details |
|---|
| Sequence | GGAGTGAGATGCAAGTGGCT | TCCTCAAGTGGCAGCAGATG | Start: 3707 |
| Tm | 60.036 | 60.036 | End: 3826 |
| Size | 20 | 20 | 120 |
| Primer 3 details |
|---|
| Sequence | TGCTTCACCGGAATCAACGA | CACTTACAGAGGCGCCAGAA | Start: 286 |
| Tm | 59.966 | 60.037 | End: 472 |
| Size | 20 | 20 | 187 |
| Primer 4 details |
|---|
| Sequence | GGAGGATGGGGCACAAGAAA | AGACTTATCGGCTGGGGAGT | Start: 4299 |
| Tm | 59.96 | 60.032 | End: 4483 |
| Size | 20 | 20 | 185 |
| Primer 5 details |
|---|
| Sequence | GCTCGAGTCTTCCACGTCAA | GGTCCAGCAGAGTTTCGTGA | Start: 4859 |
| Tm | 60.039 | 59.966 | End: 5043 |
| Size | 20 | 20 | 185 |
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