Primer Details for TRINITY_DN13350_c4_g1_i1 | |||
|---|---|---|---|
| (A)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGACAGTCACGGAAGAAACT | AGAAGTGTTTGGTAACGTTTCATGT | Start: 81 |
| Tm | 57.463 | 59.644 | End: 187 |
| Size | 21 | 25 | 107 |
| Primer 2 details | |||
| Sequence | TTGACAGTCACGGAAGAAACT | AGTGTTTGGTAACGTTTCATGTT | Start: 81 |
| Tm | 57.463 | 57.43 | End: 184 |
| Size | 21 | 23 | 104 |
| Primer 3 details | |||
| Sequence | TTGACAGTCACGGAAGAAACT | AAGTGTTTGGTAACGTTTCATGT | Start: 81 |
| Tm | 57.463 | 57.43 | End: 185 |
| Size | 21 | 23 | 105 |
| Primer 4 details | |||
| Sequence | TTGACAGTCACGGAAGAAACT | GAAGTGTTTGGTAACGTTTCATGT | Start: 81 |
| Tm | 57.463 | 58.425 | End: 186 |
| Size | 21 | 24 | 106 |
| Primer 5 details | |||
| Sequence | TTGACAGTCACGGAAGAAACT | AGAAGTGTTTGGTAACGTTTCATG | Start: 81 |
| Tm | 57.463 | 58.183 | End: 187 |
| Size | 21 | 24 | 107 |
