ssr_primer
Primer Details for TRINITY_DN13344_c2_g1_i1 |
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| (AAG)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGGATCCAAACAACTGGT | GGAGAAATGGGGAGCTGGAG | Start: 54 |
| Tm | 59.523 | 59.818 | End: 207 |
| Size | 20 | 20 | 154 |
| Primer 2 details |
|---|
| Sequence | ACGGGATCCAAACAACTGGT | AGGAGAAATGGGGAGCTGGA | Start: 54 |
| Tm | 59.523 | 60.253 | End: 208 |
| Size | 20 | 20 | 155 |
| Primer 3 details |
|---|
| Sequence | ACGGGATCCAAACAACTGGT | GAAATGGGGAGCTGGAGTCG | Start: 54 |
| Tm | 59.523 | 60.463 | End: 204 |
| Size | 20 | 20 | 151 |
| Primer 4 details |
|---|
| Sequence | CGCGTCTAAATGCAGGAGCT | GGAGAAATGGGGAGCTGGAG | Start: 19 |
| Tm | 60.808 | 59.818 | End: 207 |
| Size | 20 | 20 | 189 |
| Primer 5 details |
|---|
| Sequence | CGCGTCTAAATGCAGGAGCT | AGGAGAAATGGGGAGCTGGA | Start: 19 |
| Tm | 60.808 | 60.253 | End: 208 |
| Size | 20 | 20 | 190 |
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