ssr_primer
Primer Details for TRINITY_DN13313_c4_g2_i5 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGTGGATTGGGCTGAATG | ATGAGGCAGCTACGGTCATG | Start: 4773 |
| Tm | 60.033 | 59.894 | End: 4874 |
| Size | 20 | 20 | 102 |
| Primer 2 details |
|---|
| Sequence | TCACTGCCACTTTCTGTCCC | AGGCATCTTGAACCATGGGG | Start: 435 |
| Tm | 59.891 | 60.033 | End: 605 |
| Size | 20 | 20 | 171 |
| Primer 3 details |
|---|
| Sequence | TCACTGCCACTTTCTGTCCC | GGCATCTTGAACCATGGGGA | Start: 435 |
| Tm | 59.891 | 60.033 | End: 604 |
| Size | 20 | 20 | 170 |
| Primer 4 details |
|---|
| Sequence | CACTGCCACTTTCTGTCCCT | AGGCATCTTGAACCATGGGG | Start: 436 |
| Tm | 59.891 | 60.033 | End: 605 |
| Size | 20 | 20 | 170 |
| Primer 5 details |
|---|
| Sequence | CACTGCCACTTTCTGTCCCT | GGCATCTTGAACCATGGGGA | Start: 436 |
| Tm | 59.891 | 60.033 | End: 604 |
| Size | 20 | 20 | 169 |
(GAA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGTGGATTGGGCTGAATG | ATGAGGCAGCTACGGTCATG | Start: 4773 |
| Tm | 60.033 | 59.894 | End: 4874 |
| Size | 20 | 20 | 102 |
| Primer 2 details |
|---|
| Sequence | TCACTGCCACTTTCTGTCCC | AGGCATCTTGAACCATGGGG | Start: 435 |
| Tm | 59.891 | 60.033 | End: 605 |
| Size | 20 | 20 | 171 |
| Primer 3 details |
|---|
| Sequence | TCACTGCCACTTTCTGTCCC | GGCATCTTGAACCATGGGGA | Start: 435 |
| Tm | 59.891 | 60.033 | End: 604 |
| Size | 20 | 20 | 170 |
| Primer 4 details |
|---|
| Sequence | CACTGCCACTTTCTGTCCCT | AGGCATCTTGAACCATGGGG | Start: 436 |
| Tm | 59.891 | 60.033 | End: 605 |
| Size | 20 | 20 | 170 |
| Primer 5 details |
|---|
| Sequence | CACTGCCACTTTCTGTCCCT | GGCATCTTGAACCATGGGGA | Start: 436 |
| Tm | 59.891 | 60.033 | End: 604 |
| Size | 20 | 20 | 169 |
(A)11gaaaaagaaaaacaaatctcaattttcgaaatcc(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGTGGATTGGGCTGAATG | ATGAGGCAGCTACGGTCATG | Start: 4773 |
| Tm | 60.033 | 59.894 | End: 4874 |
| Size | 20 | 20 | 102 |
| Primer 2 details |
|---|
| Sequence | TCACTGCCACTTTCTGTCCC | AGGCATCTTGAACCATGGGG | Start: 435 |
| Tm | 59.891 | 60.033 | End: 605 |
| Size | 20 | 20 | 171 |
| Primer 3 details |
|---|
| Sequence | TCACTGCCACTTTCTGTCCC | GGCATCTTGAACCATGGGGA | Start: 435 |
| Tm | 59.891 | 60.033 | End: 604 |
| Size | 20 | 20 | 170 |
| Primer 4 details |
|---|
| Sequence | CACTGCCACTTTCTGTCCCT | AGGCATCTTGAACCATGGGG | Start: 436 |
| Tm | 59.891 | 60.033 | End: 605 |
| Size | 20 | 20 | 170 |
| Primer 5 details |
|---|
| Sequence | CACTGCCACTTTCTGTCCCT | GGCATCTTGAACCATGGGGA | Start: 436 |
| Tm | 59.891 | 60.033 | End: 604 |
| Size | 20 | 20 | 169 |
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