Primer Details for TRINITY_DN13312_c4_g1_i1 |
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| (A)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAGAACTTGGGATCTCTCTCCA | TGAAATGTGATTGAGGTAGTTGTGT | Start: 28 |
| Tm | 59.988 | 58.939 | End: 132 |
| Size | 23 | 25 | 105 |
| Primer 2 details |
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| Sequence | GAGAACTTGGGATCTCTCTCCA | TGAAATGTGATTGAGGTAGTTGTGT | Start: 29 |
| Tm | 58.63 | 58.939 | End: 132 |
| Size | 22 | 25 | 104 |
| Primer 3 details |
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| Sequence | AGAACTTGGGATCTCTCTCCA | TGAAATGTGATTGAGGTAGTTGTGT | Start: 30 |
| Tm | 57.518 | 58.939 | End: 132 |
| Size | 21 | 25 | 103 |
| Primer 4 details |
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| Sequence | AGAGAACTTGGGATCTCTCTCCA | TGAAATGTGATTGAGGTAGTTGTGTT | Start: 28 |
| Tm | 59.988 | 59.454 | End: 132 |
| Size | 23 | 26 | 105 |
| Primer 5 details |
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| Sequence | AGAGAACTTGGGATCTCTCTCCA | TTGAAATGTGATTGAGGTAGTTGTGT | Start: 28 |
| Tm | 59.988 | 59.454 | End: 133 |
| Size | 23 | 26 | 106 |
