ssr_primer
Primer Details for TRINITY_DN13311_c2_g6_i1 |
|---|
| (TCT)7tcaattctctctctg(T)11ctttagcatttgatgactgtggtcctcaaacaaggtaccataatcaaaaccctttacaattttttctatgtaattaaccct(TTC)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGGTGGAAACAATGACGGC | ACCACCGCCAAGATTGTGAT | Start: 1287 |
| Tm | 60.04 | 59.962 | End: 1396 |
| Size | 20 | 20 | 110 |
| Primer 2 details |
|---|
| Sequence | TTTTCAAGGAGCTCCCGGAC | CGGCGGAGGATATGGATACG | Start: 1762 |
| Tm | 59.964 | 59.899 | End: 1942 |
| Size | 20 | 20 | 181 |
| Primer 3 details |
|---|
| Sequence | AGACGTTTGTGGGTGGTACC | CCGCCTTTCTTCCCTCCATT | Start: 1065 |
| Tm | 59.893 | 60.034 | End: 1335 |
| Size | 20 | 20 | 271 |
| Primer 4 details |
|---|
| Sequence | CCACCGCAGTTGAAGGGTAT | GGTACCACCCACAAACGTCT | Start: 860 |
| Tm | 60.035 | 59.893 | End: 1084 |
| Size | 20 | 20 | 225 |
| Primer 5 details |
|---|
| Sequence | AATGGAGGGAAGAAAGGCGG | CAATGCTCGGAAAACCGTGG | Start: 1316 |
| Tm | 60.034 | 60.11 | End: 1565 |
| Size | 20 | 20 | 250 |
(GGT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGGTGGAAACAATGACGGC | ACCACCGCCAAGATTGTGAT | Start: 1287 |
| Tm | 60.04 | 59.962 | End: 1396 |
| Size | 20 | 20 | 110 |
| Primer 2 details |
|---|
| Sequence | TTTTCAAGGAGCTCCCGGAC | CGGCGGAGGATATGGATACG | Start: 1762 |
| Tm | 59.964 | 59.899 | End: 1942 |
| Size | 20 | 20 | 181 |
| Primer 3 details |
|---|
| Sequence | AGACGTTTGTGGGTGGTACC | CCGCCTTTCTTCCCTCCATT | Start: 1065 |
| Tm | 59.893 | 60.034 | End: 1335 |
| Size | 20 | 20 | 271 |
| Primer 4 details |
|---|
| Sequence | CCACCGCAGTTGAAGGGTAT | GGTACCACCCACAAACGTCT | Start: 860 |
| Tm | 60.035 | 59.893 | End: 1084 |
| Size | 20 | 20 | 225 |
| Primer 5 details |
|---|
| Sequence | AATGGAGGGAAGAAAGGCGG | CAATGCTCGGAAAACCGTGG | Start: 1316 |
| Tm | 60.034 | 60.11 | End: 1565 |
| Size | 20 | 20 | 250 |
(GAT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGGTGGAAACAATGACGGC | ACCACCGCCAAGATTGTGAT | Start: 1287 |
| Tm | 60.04 | 59.962 | End: 1396 |
| Size | 20 | 20 | 110 |
| Primer 2 details |
|---|
| Sequence | TTTTCAAGGAGCTCCCGGAC | CGGCGGAGGATATGGATACG | Start: 1762 |
| Tm | 59.964 | 59.899 | End: 1942 |
| Size | 20 | 20 | 181 |
| Primer 3 details |
|---|
| Sequence | AGACGTTTGTGGGTGGTACC | CCGCCTTTCTTCCCTCCATT | Start: 1065 |
| Tm | 59.893 | 60.034 | End: 1335 |
| Size | 20 | 20 | 271 |
| Primer 4 details |
|---|
| Sequence | CCACCGCAGTTGAAGGGTAT | GGTACCACCCACAAACGTCT | Start: 860 |
| Tm | 60.035 | 59.893 | End: 1084 |
| Size | 20 | 20 | 225 |
| Primer 5 details |
|---|
| Sequence | AATGGAGGGAAGAAAGGCGG | CAATGCTCGGAAAACCGTGG | Start: 1316 |
| Tm | 60.034 | 60.11 | End: 1565 |
| Size | 20 | 20 | 250 |
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