ssr_primer
Primer Details for TRINITY_DN13311_c2_g3_i4 |
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| (AG)8aaaaagggaggagagagagaagaagaaggaagaagaagagaaaaaagagaaaagagagagagaaatccatcggccgcctgacctcttttctcc(CTT)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAATCCCCCACCTGAAGTG | TTTGCGAGGCCGTAGTCAAT | Start: 2305 |
| Tm | 60.034 | 60.037 | End: 2576 |
| Size | 20 | 20 | 272 |
| Primer 2 details |
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| Sequence | TGGAATTACCCTGCCGGAAC | GCTTGACAGCAACCTCCTCT | Start: 581 |
| Tm | 60.035 | 59.964 | End: 819 |
| Size | 20 | 20 | 239 |
| Primer 3 details |
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| Sequence | GTGGAATTACCCTGCCGGAA | GCTTGACAGCAACCTCCTCT | Start: 580 |
| Tm | 60.035 | 59.964 | End: 819 |
| Size | 20 | 20 | 240 |
| Primer 4 details |
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| Sequence | AAGTCGTCGGCAGTGGAATT | GCTTGACAGCAACCTCCTCT | Start: 568 |
| Tm | 59.965 | 59.964 | End: 819 |
| Size | 20 | 20 | 252 |
| Primer 5 details |
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| Sequence | TCACCATCTCCCTTCCGACT | TTCCGGCAGGGTAATTCCAC | Start: 349 |
| Tm | 59.959 | 60.035 | End: 599 |
| Size | 20 | 20 | 251 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAATCCCCCACCTGAAGTG | TTTGCGAGGCCGTAGTCAAT | Start: 2305 |
| Tm | 60.034 | 60.037 | End: 2576 |
| Size | 20 | 20 | 272 |
| Primer 2 details |
|---|
| Sequence | TGGAATTACCCTGCCGGAAC | GCTTGACAGCAACCTCCTCT | Start: 581 |
| Tm | 60.035 | 59.964 | End: 819 |
| Size | 20 | 20 | 239 |
| Primer 3 details |
|---|
| Sequence | GTGGAATTACCCTGCCGGAA | GCTTGACAGCAACCTCCTCT | Start: 580 |
| Tm | 60.035 | 59.964 | End: 819 |
| Size | 20 | 20 | 240 |
| Primer 4 details |
|---|
| Sequence | AAGTCGTCGGCAGTGGAATT | GCTTGACAGCAACCTCCTCT | Start: 568 |
| Tm | 59.965 | 59.964 | End: 819 |
| Size | 20 | 20 | 252 |
| Primer 5 details |
|---|
| Sequence | TCACCATCTCCCTTCCGACT | TTCCGGCAGGGTAATTCCAC | Start: 349 |
| Tm | 59.959 | 60.035 | End: 599 |
| Size | 20 | 20 | 251 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAATCCCCCACCTGAAGTG | TTTGCGAGGCCGTAGTCAAT | Start: 2305 |
| Tm | 60.034 | 60.037 | End: 2576 |
| Size | 20 | 20 | 272 |
| Primer 2 details |
|---|
| Sequence | TGGAATTACCCTGCCGGAAC | GCTTGACAGCAACCTCCTCT | Start: 581 |
| Tm | 60.035 | 59.964 | End: 819 |
| Size | 20 | 20 | 239 |
| Primer 3 details |
|---|
| Sequence | GTGGAATTACCCTGCCGGAA | GCTTGACAGCAACCTCCTCT | Start: 580 |
| Tm | 60.035 | 59.964 | End: 819 |
| Size | 20 | 20 | 240 |
| Primer 4 details |
|---|
| Sequence | AAGTCGTCGGCAGTGGAATT | GCTTGACAGCAACCTCCTCT | Start: 568 |
| Tm | 59.965 | 59.964 | End: 819 |
| Size | 20 | 20 | 252 |
| Primer 5 details |
|---|
| Sequence | TCACCATCTCCCTTCCGACT | TTCCGGCAGGGTAATTCCAC | Start: 349 |
| Tm | 59.959 | 60.035 | End: 599 |
| Size | 20 | 20 | 251 |
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