ssr_primer
Primer Details for TRINITY_DN13306_c5_g1_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCAGGGTGGAGGATCAAAC | GGCTCGGCAAAACCTAAACA | Start: 73 |
| Tm | 59.962 | 59.044 | End: 205 |
| Size | 20 | 20 | 133 |
| Primer 2 details |
|---|
| Sequence | TGCAGGGTGGAGGATCAAAC | TGGCTCGGCAAAACCTAAAC | Start: 73 |
| Tm | 59.962 | 59.044 | End: 206 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
|---|
| Sequence | TGCAGGGTGGAGGATCAAAC | AAATTGGCTCGGCAAAACCT | Start: 73 |
| Tm | 59.962 | 58.952 | End: 210 |
| Size | 20 | 20 | 138 |
| Primer 4 details |
|---|
| Sequence | TTCAAACCTTGAAGGCCCCA | GGCTCGGCAAAACCTAAACA | Start: 93 |
| Tm | 59.737 | 59.044 | End: 205 |
| Size | 20 | 20 | 113 |
| Primer 5 details |
|---|
| Sequence | TTCAAACCTTGAAGGCCCCA | TGGCTCGGCAAAACCTAAAC | Start: 93 |
| Tm | 59.737 | 59.044 | End: 206 |
| Size | 20 | 20 | 114 |
(T)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCAGGGTGGAGGATCAAAC | GGCTCGGCAAAACCTAAACA | Start: 73 |
| Tm | 59.962 | 59.044 | End: 205 |
| Size | 20 | 20 | 133 |
| Primer 2 details |
|---|
| Sequence | TGCAGGGTGGAGGATCAAAC | TGGCTCGGCAAAACCTAAAC | Start: 73 |
| Tm | 59.962 | 59.044 | End: 206 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
|---|
| Sequence | TGCAGGGTGGAGGATCAAAC | AAATTGGCTCGGCAAAACCT | Start: 73 |
| Tm | 59.962 | 58.952 | End: 210 |
| Size | 20 | 20 | 138 |
| Primer 4 details |
|---|
| Sequence | TTCAAACCTTGAAGGCCCCA | GGCTCGGCAAAACCTAAACA | Start: 93 |
| Tm | 59.737 | 59.044 | End: 205 |
| Size | 20 | 20 | 113 |
| Primer 5 details |
|---|
| Sequence | TTCAAACCTTGAAGGCCCCA | TGGCTCGGCAAAACCTAAAC | Start: 93 |
| Tm | 59.737 | 59.044 | End: 206 |
| Size | 20 | 20 | 114 |
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