ssr_primer
Primer Details for TRINITY_DN13294_c1_g1_i6 |
|---|
| (CT)7: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCAACTCGGCTTAGAACGT | CGAGTGGAAATGGGAGCACT | Start: 3439 |
| Tm | 59.967 | 60.036 | End: 3556 |
| Size | 20 | 20 | 118 |
| Primer 2 details |
|---|
| Sequence | TTGGGTCAGACACGAGCATC | TTGGGGAATGATGGGTGACG | Start: 913 |
| Tm | 60.037 | 60.035 | End: 1030 |
| Size | 20 | 20 | 118 |
| Primer 3 details |
|---|
| Sequence | CGTTGTTGTTCGCGTGGATT | TTGGGGAATGATGGGTGACG | Start: 765 |
| Tm | 60.042 | 60.035 | End: 1030 |
| Size | 20 | 20 | 266 |
| Primer 4 details |
|---|
| Sequence | CGTTGTTGTTCGCGTGGATT | GATGCTCGTGTCTGACCCAA | Start: 765 |
| Tm | 60.042 | 60.037 | End: 932 |
| Size | 20 | 20 | 168 |
| Primer 5 details |
|---|
| Sequence | CGTCACCCATCATTCCCCAA | AGAATCCCCCATCAGTCCCA | Start: 1011 |
| Tm | 60.035 | 59.954 | End: 1243 |
| Size | 20 | 20 | 233 |
(TCT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCAACTCGGCTTAGAACGT | CGAGTGGAAATGGGAGCACT | Start: 3439 |
| Tm | 59.967 | 60.036 | End: 3556 |
| Size | 20 | 20 | 118 |
| Primer 2 details |
|---|
| Sequence | TTGGGTCAGACACGAGCATC | TTGGGGAATGATGGGTGACG | Start: 913 |
| Tm | 60.037 | 60.035 | End: 1030 |
| Size | 20 | 20 | 118 |
| Primer 3 details |
|---|
| Sequence | CGTTGTTGTTCGCGTGGATT | TTGGGGAATGATGGGTGACG | Start: 765 |
| Tm | 60.042 | 60.035 | End: 1030 |
| Size | 20 | 20 | 266 |
| Primer 4 details |
|---|
| Sequence | CGTTGTTGTTCGCGTGGATT | GATGCTCGTGTCTGACCCAA | Start: 765 |
| Tm | 60.042 | 60.037 | End: 932 |
| Size | 20 | 20 | 168 |
| Primer 5 details |
|---|
| Sequence | CGTCACCCATCATTCCCCAA | AGAATCCCCCATCAGTCCCA | Start: 1011 |
| Tm | 60.035 | 59.954 | End: 1243 |
| Size | 20 | 20 | 233 |
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