ssr_primer
Primer Details for TRINITY_DN13288_c1_g6_i1 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAATTGGTGGCTGTCCGTA | AAGACGCTGACGGTCACAAT | Start: 203 |
| Tm | 60.036 | 59.966 | End: 435 |
| Size | 20 | 20 | 233 |
| Primer 2 details |
|---|
| Sequence | ATTGTGACCGTCAGCGTCTT | GTCAGAGCAAAGCACCAACG | Start: 416 |
| Tm | 59.966 | 60.041 | End: 539 |
| Size | 20 | 20 | 124 |
| Primer 3 details |
|---|
| Sequence | ATTGTGACCGTCAGCGTCTT | CGTCAGAGCAAAGCACCAAC | Start: 416 |
| Tm | 59.966 | 60.041 | End: 540 |
| Size | 20 | 20 | 125 |
| Primer 4 details |
|---|
| Sequence | ACGCACCTGACAATGACAGT | CCAAGCCAAACCAGAAACCG | Start: 348 |
| Tm | 59.893 | 59.968 | End: 491 |
| Size | 20 | 20 | 144 |
| Primer 5 details |
|---|
| Sequence | GGCCAGGCAGTGAGATGATT | CCAAGCCAAACCAGAAACCG | Start: 308 |
| Tm | 60.107 | 59.968 | End: 491 |
| Size | 20 | 20 | 184 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAATTGGTGGCTGTCCGTA | AAGACGCTGACGGTCACAAT | Start: 203 |
| Tm | 60.036 | 59.966 | End: 435 |
| Size | 20 | 20 | 233 |
| Primer 2 details |
|---|
| Sequence | ATTGTGACCGTCAGCGTCTT | GTCAGAGCAAAGCACCAACG | Start: 416 |
| Tm | 59.966 | 60.041 | End: 539 |
| Size | 20 | 20 | 124 |
| Primer 3 details |
|---|
| Sequence | ATTGTGACCGTCAGCGTCTT | CGTCAGAGCAAAGCACCAAC | Start: 416 |
| Tm | 59.966 | 60.041 | End: 540 |
| Size | 20 | 20 | 125 |
| Primer 4 details |
|---|
| Sequence | ACGCACCTGACAATGACAGT | CCAAGCCAAACCAGAAACCG | Start: 348 |
| Tm | 59.893 | 59.968 | End: 491 |
| Size | 20 | 20 | 144 |
| Primer 5 details |
|---|
| Sequence | GGCCAGGCAGTGAGATGATT | CCAAGCCAAACCAGAAACCG | Start: 308 |
| Tm | 60.107 | 59.968 | End: 491 |
| Size | 20 | 20 | 184 |
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