ssr_primer
Primer Details for TRINITY_DN13277_c0_g5_i2 |
|---|
| (GT)8tgtgaaattaaagaaaga(AG)8: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACGATCTTCATCTCCGCGT | GGCGGGTCCAGTTCTAACAA | Start: 1351 |
| Tm | 59.97 | 59.964 | End: 1456 |
| Size | 20 | 20 | 106 |
| Primer 2 details |
|---|
| Sequence | CAACCACCCATCCTTGTCGA | ACGCGGAGATGAAGATCGTC | Start: 1219 |
| Tm | 59.964 | 59.97 | End: 1370 |
| Size | 20 | 20 | 152 |
| Primer 3 details |
|---|
| Sequence | CAACCACCCATCCTTGTCGA | GGCGGGTCCAGTTCTAACAA | Start: 1219 |
| Tm | 59.964 | 59.964 | End: 1456 |
| Size | 20 | 20 | 238 |
| Primer 4 details |
|---|
| Sequence | GACGATCTTCATCTCCGCGT | GAGGCGGGTCCAGTTCTAAC | Start: 1351 |
| Tm | 59.97 | 60.108 | End: 1458 |
| Size | 20 | 20 | 108 |
| Primer 5 details |
|---|
| Sequence | ATCCAATATCCCCGGTGTGC | ACGCGGAGATGAAGATCGTC | Start: 1200 |
| Tm | 59.89 | 59.97 | End: 1370 |
| Size | 20 | 20 | 171 |
(AT)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACGATCTTCATCTCCGCGT | GGCGGGTCCAGTTCTAACAA | Start: 1351 |
| Tm | 59.97 | 59.964 | End: 1456 |
| Size | 20 | 20 | 106 |
| Primer 2 details |
|---|
| Sequence | CAACCACCCATCCTTGTCGA | ACGCGGAGATGAAGATCGTC | Start: 1219 |
| Tm | 59.964 | 59.97 | End: 1370 |
| Size | 20 | 20 | 152 |
| Primer 3 details |
|---|
| Sequence | CAACCACCCATCCTTGTCGA | GGCGGGTCCAGTTCTAACAA | Start: 1219 |
| Tm | 59.964 | 59.964 | End: 1456 |
| Size | 20 | 20 | 238 |
| Primer 4 details |
|---|
| Sequence | GACGATCTTCATCTCCGCGT | GAGGCGGGTCCAGTTCTAAC | Start: 1351 |
| Tm | 59.97 | 60.108 | End: 1458 |
| Size | 20 | 20 | 108 |
| Primer 5 details |
|---|
| Sequence | ATCCAATATCCCCGGTGTGC | ACGCGGAGATGAAGATCGTC | Start: 1200 |
| Tm | 59.89 | 59.97 | End: 1370 |
| Size | 20 | 20 | 171 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACGATCTTCATCTCCGCGT | GGCGGGTCCAGTTCTAACAA | Start: 1351 |
| Tm | 59.97 | 59.964 | End: 1456 |
| Size | 20 | 20 | 106 |
| Primer 2 details |
|---|
| Sequence | CAACCACCCATCCTTGTCGA | ACGCGGAGATGAAGATCGTC | Start: 1219 |
| Tm | 59.964 | 59.97 | End: 1370 |
| Size | 20 | 20 | 152 |
| Primer 3 details |
|---|
| Sequence | CAACCACCCATCCTTGTCGA | GGCGGGTCCAGTTCTAACAA | Start: 1219 |
| Tm | 59.964 | 59.964 | End: 1456 |
| Size | 20 | 20 | 238 |
| Primer 4 details |
|---|
| Sequence | GACGATCTTCATCTCCGCGT | GAGGCGGGTCCAGTTCTAAC | Start: 1351 |
| Tm | 59.97 | 60.108 | End: 1458 |
| Size | 20 | 20 | 108 |
| Primer 5 details |
|---|
| Sequence | ATCCAATATCCCCGGTGTGC | ACGCGGAGATGAAGATCGTC | Start: 1200 |
| Tm | 59.89 | 59.97 | End: 1370 |
| Size | 20 | 20 | 171 |
(A)19ctgttttttgaaaattgttttttt(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACGATCTTCATCTCCGCGT | GGCGGGTCCAGTTCTAACAA | Start: 1351 |
| Tm | 59.97 | 59.964 | End: 1456 |
| Size | 20 | 20 | 106 |
| Primer 2 details |
|---|
| Sequence | CAACCACCCATCCTTGTCGA | ACGCGGAGATGAAGATCGTC | Start: 1219 |
| Tm | 59.964 | 59.97 | End: 1370 |
| Size | 20 | 20 | 152 |
| Primer 3 details |
|---|
| Sequence | CAACCACCCATCCTTGTCGA | GGCGGGTCCAGTTCTAACAA | Start: 1219 |
| Tm | 59.964 | 59.964 | End: 1456 |
| Size | 20 | 20 | 238 |
| Primer 4 details |
|---|
| Sequence | GACGATCTTCATCTCCGCGT | GAGGCGGGTCCAGTTCTAAC | Start: 1351 |
| Tm | 59.97 | 60.108 | End: 1458 |
| Size | 20 | 20 | 108 |
| Primer 5 details |
|---|
| Sequence | ATCCAATATCCCCGGTGTGC | ACGCGGAGATGAAGATCGTC | Start: 1200 |
| Tm | 59.89 | 59.97 | End: 1370 |
| Size | 20 | 20 | 171 |
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