ssr_primer
Primer Details for TRINITY_DN13265_c2_g3_i4 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATTTCCCCACGGCATTTGC | TCCACACACACACACACACA | Start: 669 |
| Tm | 60.036 | 59.965 | End: 838 |
| Size | 20 | 20 | 170 |
| Primer 2 details |
|---|
| Sequence | GCTGTCAAAGGCAACCCATG | TCCACACACACACACACACA | Start: 596 |
| Tm | 60.038 | 59.965 | End: 838 |
| Size | 20 | 20 | 243 |
| Primer 3 details |
|---|
| Sequence | CCTCCCTCACAATGCACCAT | CAACGCGAACAGTGGCATAG | Start: 3219 |
| Tm | 60.034 | 59.905 | End: 3493 |
| Size | 20 | 20 | 275 |
| Primer 4 details |
|---|
| Sequence | TGTCAAAGGCAACCCATGGT | TCCACACACACACACACACA | Start: 598 |
| Tm | 60.106 | 59.965 | End: 838 |
| Size | 20 | 20 | 241 |
| Primer 5 details |
|---|
| Sequence | AGCCCCATGAACAACGGTAG | GTTTATGGCCCTTGTGCTGC | Start: 2666 |
| Tm | 60.035 | 60.109 | End: 2812 |
| Size | 20 | 20 | 147 |
(TG)6tttgtgtcaaaggcacccatggtgttatggtgctagcatatattcttcctttgttccatcattgatattgtctggcatttgccttcactat(TG)15gag(GA)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATTTCCCCACGGCATTTGC | TCCACACACACACACACACA | Start: 669 |
| Tm | 60.036 | 59.965 | End: 838 |
| Size | 20 | 20 | 170 |
| Primer 2 details |
|---|
| Sequence | GCTGTCAAAGGCAACCCATG | TCCACACACACACACACACA | Start: 596 |
| Tm | 60.038 | 59.965 | End: 838 |
| Size | 20 | 20 | 243 |
| Primer 3 details |
|---|
| Sequence | CCTCCCTCACAATGCACCAT | CAACGCGAACAGTGGCATAG | Start: 3219 |
| Tm | 60.034 | 59.905 | End: 3493 |
| Size | 20 | 20 | 275 |
| Primer 4 details |
|---|
| Sequence | TGTCAAAGGCAACCCATGGT | TCCACACACACACACACACA | Start: 598 |
| Tm | 60.106 | 59.965 | End: 838 |
| Size | 20 | 20 | 241 |
| Primer 5 details |
|---|
| Sequence | AGCCCCATGAACAACGGTAG | GTTTATGGCCCTTGTGCTGC | Start: 2666 |
| Tm | 60.035 | 60.109 | End: 2812 |
| Size | 20 | 20 | 147 |
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