ssr_primer
Primer Details for TRINITY_DN13254_c1_g1_i3 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCCCCAAACCTTCGACTCC | CCACCCCTTTTGAAGCGTTG | Start: 1810 |
| Tm | 60.036 | 59.968 | End: 1966 |
| Size | 20 | 20 | 157 |
| Primer 2 details |
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| Sequence | CCACTTTCCAGGGGCATCAT | ACTGGCGGCTTAATGTTCCA | Start: 2850 |
| Tm | 60.033 | 59.962 | End: 2983 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
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| Sequence | TGGAACCCACTGCCGAATAC | CAATTCAGCTCCCAGCAGGA | Start: 972 |
| Tm | 60.036 | 60.035 | End: 1117 |
| Size | 20 | 20 | 146 |
| Primer 4 details |
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| Sequence | TCTGCATTGTCAGGCATGGT | CCACCCCTTTTGAAGCGTTG | Start: 1693 |
| Tm | 59.961 | 59.968 | End: 1966 |
| Size | 20 | 20 | 274 |
| Primer 5 details |
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| Sequence | ACAGATTGAGCAGACCGCAA | CACCAACTGGAGCTACAGCA | Start: 3885 |
| Tm | 59.965 | 59.965 | End: 4116 |
| Size | 20 | 20 | 232 |
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