ssr_primer
Primer Details for TRINITY_DN13247_c2_g1_i2 |
|---|
| (A)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACTGCCACCAAACAGGAA | CGTCCATGGTGGTCTGTCTC | Start: 1040 |
| Tm | 60.034 | 60.109 | End: 1219 |
| Size | 20 | 20 | 180 |
| Primer 2 details |
|---|
| Sequence | AACACTGCCACCAAACAGGA | CGTCCATGGTGGTCTGTCTC | Start: 1039 |
| Tm | 60.034 | 60.109 | End: 1219 |
| Size | 20 | 20 | 181 |
| Primer 3 details |
|---|
| Sequence | ACACTGCCACCAAACAGGAA | TGGCTCCGTGAATGTTTGGA | Start: 1040 |
| Tm | 60.034 | 59.89 | End: 1303 |
| Size | 20 | 20 | 264 |
| Primer 4 details |
|---|
| Sequence | AACACTGCCACCAAACAGGA | TGGCTCCGTGAATGTTTGGA | Start: 1039 |
| Tm | 60.034 | 59.89 | End: 1303 |
| Size | 20 | 20 | 265 |
| Primer 5 details |
|---|
| Sequence | AGTCACAACACTGCCACCAA | CGTCCATGGTGGTCTGTCTC | Start: 1033 |
| Tm | 60.035 | 60.109 | End: 1219 |
| Size | 20 | 20 | 187 |
(TCTTT)7tttcttttttc(T)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACTGCCACCAAACAGGAA | CGTCCATGGTGGTCTGTCTC | Start: 1040 |
| Tm | 60.034 | 60.109 | End: 1219 |
| Size | 20 | 20 | 180 |
| Primer 2 details |
|---|
| Sequence | AACACTGCCACCAAACAGGA | CGTCCATGGTGGTCTGTCTC | Start: 1039 |
| Tm | 60.034 | 60.109 | End: 1219 |
| Size | 20 | 20 | 181 |
| Primer 3 details |
|---|
| Sequence | ACACTGCCACCAAACAGGAA | TGGCTCCGTGAATGTTTGGA | Start: 1040 |
| Tm | 60.034 | 59.89 | End: 1303 |
| Size | 20 | 20 | 264 |
| Primer 4 details |
|---|
| Sequence | AACACTGCCACCAAACAGGA | TGGCTCCGTGAATGTTTGGA | Start: 1039 |
| Tm | 60.034 | 59.89 | End: 1303 |
| Size | 20 | 20 | 265 |
| Primer 5 details |
|---|
| Sequence | AGTCACAACACTGCCACCAA | CGTCCATGGTGGTCTGTCTC | Start: 1033 |
| Tm | 60.035 | 60.109 | End: 1219 |
| Size | 20 | 20 | 187 |
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