ssr_primer
Primer Details for TRINITY_DN13244_c1_g7_i6 |
|---|
| (T)11acagaacagaagatggaacaacc(A)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTCGGATCTCCATGCTCTG | TCGGCAGTGGTTAAGGACAC | Start: 1911 |
| Tm | 59.967 | 59.966 | End: 2129 |
| Size | 20 | 20 | 219 |
| Primer 2 details |
|---|
| Sequence | GTGTCCTTAACCACTGCCGA | TTGTACCCTGTCCAAGTCGC | Start: 2110 |
| Tm | 59.966 | 59.966 | End: 2272 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
|---|
| Sequence | GCGACTTGGACAGGGTACAA | GGAGCACCATTCTCCTGCTT | Start: 2253 |
| Tm | 59.966 | 60.035 | End: 2457 |
| Size | 20 | 20 | 205 |
| Primer 4 details |
|---|
| Sequence | AGTGTCCTTAACCACTGCCG | TTGTACCCTGTCCAAGTCGC | Start: 2109 |
| Tm | 59.965 | 59.966 | End: 2272 |
| Size | 20 | 20 | 164 |
| Primer 5 details |
|---|
| Sequence | ATTCACGGCGAGGGTTTCAT | AGGTAACCGACTAGCCGAGT | Start: 1422 |
| Tm | 60.035 | 60.035 | End: 1551 |
| Size | 20 | 20 | 130 |
(TGC)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTCGGATCTCCATGCTCTG | TCGGCAGTGGTTAAGGACAC | Start: 1911 |
| Tm | 59.967 | 59.966 | End: 2129 |
| Size | 20 | 20 | 219 |
| Primer 2 details |
|---|
| Sequence | GTGTCCTTAACCACTGCCGA | TTGTACCCTGTCCAAGTCGC | Start: 2110 |
| Tm | 59.966 | 59.966 | End: 2272 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
|---|
| Sequence | GCGACTTGGACAGGGTACAA | GGAGCACCATTCTCCTGCTT | Start: 2253 |
| Tm | 59.966 | 60.035 | End: 2457 |
| Size | 20 | 20 | 205 |
| Primer 4 details |
|---|
| Sequence | AGTGTCCTTAACCACTGCCG | TTGTACCCTGTCCAAGTCGC | Start: 2109 |
| Tm | 59.965 | 59.966 | End: 2272 |
| Size | 20 | 20 | 164 |
| Primer 5 details |
|---|
| Sequence | ATTCACGGCGAGGGTTTCAT | AGGTAACCGACTAGCCGAGT | Start: 1422 |
| Tm | 60.035 | 60.035 | End: 1551 |
| Size | 20 | 20 | 130 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTCGGATCTCCATGCTCTG | TCGGCAGTGGTTAAGGACAC | Start: 1911 |
| Tm | 59.967 | 59.966 | End: 2129 |
| Size | 20 | 20 | 219 |
| Primer 2 details |
|---|
| Sequence | GTGTCCTTAACCACTGCCGA | TTGTACCCTGTCCAAGTCGC | Start: 2110 |
| Tm | 59.966 | 59.966 | End: 2272 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
|---|
| Sequence | GCGACTTGGACAGGGTACAA | GGAGCACCATTCTCCTGCTT | Start: 2253 |
| Tm | 59.966 | 60.035 | End: 2457 |
| Size | 20 | 20 | 205 |
| Primer 4 details |
|---|
| Sequence | AGTGTCCTTAACCACTGCCG | TTGTACCCTGTCCAAGTCGC | Start: 2109 |
| Tm | 59.965 | 59.966 | End: 2272 |
| Size | 20 | 20 | 164 |
| Primer 5 details |
|---|
| Sequence | ATTCACGGCGAGGGTTTCAT | AGGTAACCGACTAGCCGAGT | Start: 1422 |
| Tm | 60.035 | 60.035 | End: 1551 |
| Size | 20 | 20 | 130 |
(AG)8gtagctcagagatagattagaagaagaaattg(A)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTCGGATCTCCATGCTCTG | TCGGCAGTGGTTAAGGACAC | Start: 1911 |
| Tm | 59.967 | 59.966 | End: 2129 |
| Size | 20 | 20 | 219 |
| Primer 2 details |
|---|
| Sequence | GTGTCCTTAACCACTGCCGA | TTGTACCCTGTCCAAGTCGC | Start: 2110 |
| Tm | 59.966 | 59.966 | End: 2272 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
|---|
| Sequence | GCGACTTGGACAGGGTACAA | GGAGCACCATTCTCCTGCTT | Start: 2253 |
| Tm | 59.966 | 60.035 | End: 2457 |
| Size | 20 | 20 | 205 |
| Primer 4 details |
|---|
| Sequence | AGTGTCCTTAACCACTGCCG | TTGTACCCTGTCCAAGTCGC | Start: 2109 |
| Tm | 59.965 | 59.966 | End: 2272 |
| Size | 20 | 20 | 164 |
| Primer 5 details |
|---|
| Sequence | ATTCACGGCGAGGGTTTCAT | AGGTAACCGACTAGCCGAGT | Start: 1422 |
| Tm | 60.035 | 60.035 | End: 1551 |
| Size | 20 | 20 | 130 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTCGGATCTCCATGCTCTG | TCGGCAGTGGTTAAGGACAC | Start: 1911 |
| Tm | 59.967 | 59.966 | End: 2129 |
| Size | 20 | 20 | 219 |
| Primer 2 details |
|---|
| Sequence | GTGTCCTTAACCACTGCCGA | TTGTACCCTGTCCAAGTCGC | Start: 2110 |
| Tm | 59.966 | 59.966 | End: 2272 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
|---|
| Sequence | GCGACTTGGACAGGGTACAA | GGAGCACCATTCTCCTGCTT | Start: 2253 |
| Tm | 59.966 | 60.035 | End: 2457 |
| Size | 20 | 20 | 205 |
| Primer 4 details |
|---|
| Sequence | AGTGTCCTTAACCACTGCCG | TTGTACCCTGTCCAAGTCGC | Start: 2109 |
| Tm | 59.965 | 59.966 | End: 2272 |
| Size | 20 | 20 | 164 |
| Primer 5 details |
|---|
| Sequence | ATTCACGGCGAGGGTTTCAT | AGGTAACCGACTAGCCGAGT | Start: 1422 |
| Tm | 60.035 | 60.035 | End: 1551 |
| Size | 20 | 20 | 130 |
(AG)8: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTCGGATCTCCATGCTCTG | TCGGCAGTGGTTAAGGACAC | Start: 1911 |
| Tm | 59.967 | 59.966 | End: 2129 |
| Size | 20 | 20 | 219 |
| Primer 2 details |
|---|
| Sequence | GTGTCCTTAACCACTGCCGA | TTGTACCCTGTCCAAGTCGC | Start: 2110 |
| Tm | 59.966 | 59.966 | End: 2272 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
|---|
| Sequence | GCGACTTGGACAGGGTACAA | GGAGCACCATTCTCCTGCTT | Start: 2253 |
| Tm | 59.966 | 60.035 | End: 2457 |
| Size | 20 | 20 | 205 |
| Primer 4 details |
|---|
| Sequence | AGTGTCCTTAACCACTGCCG | TTGTACCCTGTCCAAGTCGC | Start: 2109 |
| Tm | 59.965 | 59.966 | End: 2272 |
| Size | 20 | 20 | 164 |
| Primer 5 details |
|---|
| Sequence | ATTCACGGCGAGGGTTTCAT | AGGTAACCGACTAGCCGAGT | Start: 1422 |
| Tm | 60.035 | 60.035 | End: 1551 |
| Size | 20 | 20 | 130 |
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