Primer Details for TRINITY_DN13242_c0_g2_i1 |
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| (T)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTTGTTTTGAAATTTTCAGTCTCTGT | TGTCTCCATAAAAGACATTGCACA | Start: 64 |
| Tm | 58.693 | 58.931 | End: 211 |
| Size | 27 | 24 | 148 |
| Primer 2 details |
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| Sequence | GTTGTTTTGAAATTTTCAGTCTCTGT | TGTCTCCATAAAAGACATTGCACA | Start: 65 |
| Tm | 57.534 | 58.931 | End: 211 |
| Size | 26 | 24 | 147 |
| Primer 3 details |
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| Sequence | TGAGTTGTTTTGAAATTTTCAGTCTCT | TGTCTCCATAAAAGACATTGCACA | Start: 62 |
| Tm | 58.477 | 58.931 | End: 211 |
| Size | 27 | 24 | 150 |
| Primer 4 details |
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| Sequence | TGAGTTGTTTTGAAATTTTCAGTCTC | TGTCTCCATAAAAGACATTGCACA | Start: 62 |
| Tm | 57.312 | 58.931 | End: 211 |
| Size | 26 | 24 | 150 |
| Primer 5 details |
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| Sequence | AGTTGTTTTGAAATTTTCAGTCTCTG | TGTCTCCATAAAAGACATTGCACA | Start: 64 |
| Tm | 57.306 | 58.931 | End: 211 |
| Size | 26 | 24 | 148 |
