ssr_primer
Primer Details for TRINITY_DN13239_c0_g1_i16 |
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| (AC)6atcaccccaccaccatcatcatcatcaacaacacaaatattttttcaaacttatctacgatccaaacggctattcttctcttc(T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGTAGATTCGTTCTCGCGA | CCGGCAGACATGGATACCTC | Start: 1038 |
| Tm | 59.973 | 59.966 | End: 1260 |
| Size | 20 | 20 | 223 |
| Primer 2 details |
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| Sequence | GGAGCAACACAGTCGTCGTA | ACACCGGAGCTAATGGTTGG | Start: 4811 |
| Tm | 60.04 | 60.035 | End: 4962 |
| Size | 20 | 20 | 152 |
| Primer 3 details |
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| Sequence | CCGTAGATTCGTTCTCGCGA | TCGTCATACCGGCAGACATG | Start: 1038 |
| Tm | 59.973 | 59.898 | End: 1268 |
| Size | 20 | 20 | 231 |
| Primer 4 details |
|---|
| Sequence | CCGTAGATTCGTTCTCGCGA | GTCGTCATACCGGCAGACAT | Start: 1038 |
| Tm | 59.973 | 59.898 | End: 1269 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | CAGACTCCGCAAGATCAGCA | CGTCGAGTCACTTGTGACCA | Start: 3498 |
| Tm | 60.108 | 59.97 | End: 3747 |
| Size | 20 | 20 | 250 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGTAGATTCGTTCTCGCGA | CCGGCAGACATGGATACCTC | Start: 1038 |
| Tm | 59.973 | 59.966 | End: 1260 |
| Size | 20 | 20 | 223 |
| Primer 2 details |
|---|
| Sequence | GGAGCAACACAGTCGTCGTA | ACACCGGAGCTAATGGTTGG | Start: 4811 |
| Tm | 60.04 | 60.035 | End: 4962 |
| Size | 20 | 20 | 152 |
| Primer 3 details |
|---|
| Sequence | CCGTAGATTCGTTCTCGCGA | TCGTCATACCGGCAGACATG | Start: 1038 |
| Tm | 59.973 | 59.898 | End: 1268 |
| Size | 20 | 20 | 231 |
| Primer 4 details |
|---|
| Sequence | CCGTAGATTCGTTCTCGCGA | GTCGTCATACCGGCAGACAT | Start: 1038 |
| Tm | 59.973 | 59.898 | End: 1269 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | CAGACTCCGCAAGATCAGCA | CGTCGAGTCACTTGTGACCA | Start: 3498 |
| Tm | 60.108 | 59.97 | End: 3747 |
| Size | 20 | 20 | 250 |
(G)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGTAGATTCGTTCTCGCGA | CCGGCAGACATGGATACCTC | Start: 1038 |
| Tm | 59.973 | 59.966 | End: 1260 |
| Size | 20 | 20 | 223 |
| Primer 2 details |
|---|
| Sequence | GGAGCAACACAGTCGTCGTA | ACACCGGAGCTAATGGTTGG | Start: 4811 |
| Tm | 60.04 | 60.035 | End: 4962 |
| Size | 20 | 20 | 152 |
| Primer 3 details |
|---|
| Sequence | CCGTAGATTCGTTCTCGCGA | TCGTCATACCGGCAGACATG | Start: 1038 |
| Tm | 59.973 | 59.898 | End: 1268 |
| Size | 20 | 20 | 231 |
| Primer 4 details |
|---|
| Sequence | CCGTAGATTCGTTCTCGCGA | GTCGTCATACCGGCAGACAT | Start: 1038 |
| Tm | 59.973 | 59.898 | End: 1269 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | CAGACTCCGCAAGATCAGCA | CGTCGAGTCACTTGTGACCA | Start: 3498 |
| Tm | 60.108 | 59.97 | End: 3747 |
| Size | 20 | 20 | 250 |
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