Primer Details for TRINITY_DN13237_c1_g1_i1 |
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| (T)19: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGCCAACTAAGATGTTTGATCA | ACTTGATCATTCTAAAGTTCCAAACCA | Start: 127 |
| Tm | 58.44 | 59.494 | End: 227 |
| Size | 24 | 27 | 101 |
| Primer 2 details |
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| Sequence | CAGCCAACTAAGATGTTTGATCA | ACTTGATCATTCTAAAGTTCCAAACCA | Start: 128 |
| Tm | 57.115 | 59.494 | End: 227 |
| Size | 23 | 27 | 100 |
| Primer 3 details |
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| Sequence | TCAGCCAACTAAGATGTTTGATCA | CTTGATCATTCTAAAGTTCCAAACCA | Start: 127 |
| Tm | 58.44 | 58.115 | End: 226 |
| Size | 24 | 26 | 100 |
| Primer 4 details |
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| Sequence | TCAGCCAACTAAGATGTTTGATCA | ACTTGATCATTCTAAAGTTCCAAACC | Start: 127 |
| Tm | 58.44 | 58.114 | End: 227 |
| Size | 24 | 26 | 101 |
| Primer 5 details |
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| Sequence | TCAGCCAACTAAGATGTTTGATCAA | ACTTGATCATTCTAAAGTTCCAAACCA | Start: 127 |
| Tm | 58.997 | 59.494 | End: 227 |
| Size | 25 | 27 | 101 |
