ssr_primer
Primer Details for TRINITY_DN13237_c0_g2_i3 |
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| (T)15gacggacaaaatttggtaatattttaaaggccgcgccccgctgctcgtagccccacaccattcattttttcttttgacgtttt(CTC)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGATCCAGGGTTTGCATT | TGGGTTGGGCTGTTTGAACT | Start: 1638 |
| Tm | 59.96 | 60.033 | End: 1761 |
| Size | 20 | 20 | 124 |
| Primer 2 details |
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| Sequence | AATCACAAACCCAGCCAGCT | ATCGACCATTGGCAAGAGCA | Start: 1174 |
| Tm | 60.179 | 60.035 | End: 1273 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
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| Sequence | TGCTCTTGCCAATGGTCGAT | CTGGTGGGTACTGTTGCCAA | Start: 1254 |
| Tm | 60.035 | 60.179 | End: 1477 |
| Size | 20 | 20 | 224 |
| Primer 4 details |
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| Sequence | ATCACAAACCCAGCCAGCTT | TCCACATCGACCATTGGCAA | Start: 1175 |
| Tm | 60.179 | 59.963 | End: 1278 |
| Size | 20 | 20 | 104 |
| Primer 5 details |
|---|
| Sequence | AATCACAAACCCAGCCAGCT | TCCACATCGACCATTGGCAA | Start: 1174 |
| Tm | 60.179 | 59.963 | End: 1278 |
| Size | 20 | 20 | 105 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGATCCAGGGTTTGCATT | TGGGTTGGGCTGTTTGAACT | Start: 1638 |
| Tm | 59.96 | 60.033 | End: 1761 |
| Size | 20 | 20 | 124 |
| Primer 2 details |
|---|
| Sequence | AATCACAAACCCAGCCAGCT | ATCGACCATTGGCAAGAGCA | Start: 1174 |
| Tm | 60.179 | 60.035 | End: 1273 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
|---|
| Sequence | TGCTCTTGCCAATGGTCGAT | CTGGTGGGTACTGTTGCCAA | Start: 1254 |
| Tm | 60.035 | 60.179 | End: 1477 |
| Size | 20 | 20 | 224 |
| Primer 4 details |
|---|
| Sequence | ATCACAAACCCAGCCAGCTT | TCCACATCGACCATTGGCAA | Start: 1175 |
| Tm | 60.179 | 59.963 | End: 1278 |
| Size | 20 | 20 | 104 |
| Primer 5 details |
|---|
| Sequence | AATCACAAACCCAGCCAGCT | TCCACATCGACCATTGGCAA | Start: 1174 |
| Tm | 60.179 | 59.963 | End: 1278 |
| Size | 20 | 20 | 105 |
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