ssr_primers

ssr_primer

Primer Details for TRINITY_DN13235_c1_g1_i1
(GAA)7agaaagaaatttgatatttgtttcataatcagagaga(AG)7: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	GGCAGTGAAATTGGGAGGGA	TGACGCTGTTCCAACCTCTC	Start: 89
Tm	59.96	59.966	End: 268
Size	20	20	180
Primer 2 details
Sequence	AGGCAGTGAAATTGGGAGGG	TGACGCTGTTCCAACCTCTC	Start: 88
Tm	59.96	59.966	End: 268
Size	20	20	181
Primer 3 details
Sequence	GAGGGAGAGGTTGGAACAGC	ACCCCCATTGCAGTTGAGAG	Start: 245
Tm	60.036	59.961	End: 441
Size	20	20	197
Primer 4 details
Sequence	GGCAGTGAAATTGGGAGGGA	GCTGTTCCAACCTCTCCCTC	Start: 89
Tm	59.96	60.036	End: 264
Size	20	20	176
Primer 5 details
Sequence	AGGCAGTGAAATTGGGAGGG	GCTGTTCCAACCTCTCCCTC	Start: 88
Tm	59.96	60.036	End: 264
Size	20	20	177
(TCT)7: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	GGCAGTGAAATTGGGAGGGA	TGACGCTGTTCCAACCTCTC	Start: 89
Tm	59.96	59.966	End: 268
Size	20	20	180
Primer 2 details
Sequence	AGGCAGTGAAATTGGGAGGG	TGACGCTGTTCCAACCTCTC	Start: 88
Tm	59.96	59.966	End: 268
Size	20	20	181
Primer 3 details
Sequence	GAGGGAGAGGTTGGAACAGC	ACCCCCATTGCAGTTGAGAG	Start: 245
Tm	60.036	59.961	End: 441
Size	20	20	197
Primer 4 details
Sequence	GGCAGTGAAATTGGGAGGGA	GCTGTTCCAACCTCTCCCTC	Start: 89
Tm	59.96	60.036	End: 264
Size	20	20	176
Primer 5 details
Sequence	AGGCAGTGAAATTGGGAGGG	GCTGTTCCAACCTCTCCCTC	Start: 88
Tm	59.96	60.036	End: 264
Size	20	20	177
(A)10: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	GGCAGTGAAATTGGGAGGGA	TGACGCTGTTCCAACCTCTC	Start: 89
Tm	59.96	59.966	End: 268
Size	20	20	180
Primer 2 details
Sequence	AGGCAGTGAAATTGGGAGGG	TGACGCTGTTCCAACCTCTC	Start: 88
Tm	59.96	59.966	End: 268
Size	20	20	181
Primer 3 details
Sequence	GAGGGAGAGGTTGGAACAGC	ACCCCCATTGCAGTTGAGAG	Start: 245
Tm	60.036	59.961	End: 441
Size	20	20	197
Primer 4 details
Sequence	GGCAGTGAAATTGGGAGGGA	GCTGTTCCAACCTCTCCCTC	Start: 89
Tm	59.96	60.036	End: 264
Size	20	20	176
Primer 5 details
Sequence	AGGCAGTGAAATTGGGAGGG	GCTGTTCCAACCTCTCCCTC	Start: 88
Tm	59.96	60.036	End: 264
Size	20	20	177
(T)13: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	GGCAGTGAAATTGGGAGGGA	TGACGCTGTTCCAACCTCTC	Start: 89
Tm	59.96	59.966	End: 268
Size	20	20	180
Primer 2 details
Sequence	AGGCAGTGAAATTGGGAGGG	TGACGCTGTTCCAACCTCTC	Start: 88
Tm	59.96	59.966	End: 268
Size	20	20	181
Primer 3 details
Sequence	GAGGGAGAGGTTGGAACAGC	ACCCCCATTGCAGTTGAGAG	Start: 245
Tm	60.036	59.961	End: 441
Size	20	20	197
Primer 4 details
Sequence	GGCAGTGAAATTGGGAGGGA	GCTGTTCCAACCTCTCCCTC	Start: 89
Tm	59.96	60.036	End: 264
Size	20	20	176
Primer 5 details
Sequence	AGGCAGTGAAATTGGGAGGG	GCTGTTCCAACCTCTCCCTC	Start: 88
Tm	59.96	60.036	End: 264
Size	20	20	177
(T)13: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	GGCAGTGAAATTGGGAGGGA	TGACGCTGTTCCAACCTCTC	Start: 89
Tm	59.96	59.966	End: 268
Size	20	20	180
Primer 2 details
Sequence	AGGCAGTGAAATTGGGAGGG	TGACGCTGTTCCAACCTCTC	Start: 88
Tm	59.96	59.966	End: 268
Size	20	20	181
Primer 3 details
Sequence	GAGGGAGAGGTTGGAACAGC	ACCCCCATTGCAGTTGAGAG	Start: 245
Tm	60.036	59.961	End: 441
Size	20	20	197
Primer 4 details
Sequence	GGCAGTGAAATTGGGAGGGA	GCTGTTCCAACCTCTCCCTC	Start: 89
Tm	59.96	60.036	End: 264
Size	20	20	176
Primer 5 details
Sequence	AGGCAGTGAAATTGGGAGGG	GCTGTTCCAACCTCTCCCTC	Start: 88
Tm	59.96	60.036	End: 264
Size	20	20	177
(T)14ccttttaaggacttgactgttgcacttaatagagtatgatgactagattatagcaaataaa(AAT)5: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	GGCAGTGAAATTGGGAGGGA	TGACGCTGTTCCAACCTCTC	Start: 89
Tm	59.96	59.966	End: 268
Size	20	20	180
Primer 2 details
Sequence	AGGCAGTGAAATTGGGAGGG	TGACGCTGTTCCAACCTCTC	Start: 88
Tm	59.96	59.966	End: 268
Size	20	20	181
Primer 3 details
Sequence	GAGGGAGAGGTTGGAACAGC	ACCCCCATTGCAGTTGAGAG	Start: 245
Tm	60.036	59.961	End: 441
Size	20	20	197
Primer 4 details
Sequence	GGCAGTGAAATTGGGAGGGA	GCTGTTCCAACCTCTCCCTC	Start: 89
Tm	59.96	60.036	End: 264
Size	20	20	176
Primer 5 details
Sequence	AGGCAGTGAAATTGGGAGGG	GCTGTTCCAACCTCTCCCTC	Start: 88
Tm	59.96	60.036	End: 264
Size	20	20	177
(A)15: Primer 1 details
Primer Characteristics	Forward Primer	Reverse Primer	Product Details
Sequence	GGCAGTGAAATTGGGAGGGA	TGACGCTGTTCCAACCTCTC	Start: 89
Tm	59.96	59.966	End: 268
Size	20	20	180
Primer 2 details
Sequence	AGGCAGTGAAATTGGGAGGG	TGACGCTGTTCCAACCTCTC	Start: 88
Tm	59.96	59.966	End: 268
Size	20	20	181
Primer 3 details
Sequence	GAGGGAGAGGTTGGAACAGC	ACCCCCATTGCAGTTGAGAG	Start: 245
Tm	60.036	59.961	End: 441
Size	20	20	197
Primer 4 details
Sequence	GGCAGTGAAATTGGGAGGGA	GCTGTTCCAACCTCTCCCTC	Start: 89
Tm	59.96	60.036	End: 264
Size	20	20	176
Primer 5 details
Sequence	AGGCAGTGAAATTGGGAGGG	GCTGTTCCAACCTCTCCCTC	Start: 88
Tm	59.96	60.036	End: 264
Size	20	20	177

CsExSLDb: Transcriptome based Cucumis sativus Extended Shelf Life Database

Primer Details for TRINITY_DN13235_c1_g1_i1

ICAR Data Use Licence